CD16 anticorps (Biotin)

N° du produit ABIN2689004

Cet anticorps Souris Monoclonal détecte spécifiquement CD16 dans FACS. Il présente une réactivité envers Macaque rhésus, Cynomolgus et Babouin et a été mentionné dans 4+ publications.

Aperçu rapide pour CD16 anticorps (Biotin) (ABIN2689004)

Antigène: CD16

Reactivité: Macaque rhésus, Cynomolgus, Babouin

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD16 est conjugé à/à la Biotin

Application: Flow Cytometry (FACS)

Clone: 3G8

Détail du produit anti-CD16 anticorps (Biotin)

Marque: BD Pharmingen™

Attributs du produit: Reacts with the 50-65 kDa transmembrane form of IgG Fc (FcγRIII), a human NK-cell-associated antigen. CD16 is expressed on NK cells as well as macrophages and granulocytes. Reports indicate that CD16 plays a role in signal transduction and NK cell activation. CD16 antibody blocks the binding of soluble immune complexes to granulocytes. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Profile of peripheral blood lymphocytes analyzed on a FACScan

BD Pharmingen™ Biotin Mouse Anti-Human CD16 - Biotin - Clone 3G8 - Isotype Mouse IgG1, κ - Reactivity Bab, Cyno, Rhe - 100 Tests

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Isotype: IgG1 kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Volume d'échantillon: 20 μL

Restrictions: For Research Use only

Stockage

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Références pour anticorps anti-CD16 (Biotin) (ABIN2689004)

Wirthmueller, Kurosaki, Murakami, Ravetch: "Signal transduction by Fc gamma RIII (CD16) is mediated through the gamma chain." dans: The Journal of experimental medicine, Vol. 175, Issue 5, pp. 1381-90, (1992) (PubMed).

Stroncek, Skubitz, Plachta, Shankar, Clay, Herman, Fleit, McCullough: "Alloimmune neonatal neutropenia due to an antibody to the neutrophil Fc-gamma receptor III with maternal deficiency of CD16 antigen." dans: Blood, Vol. 77, Issue 7, pp. 1572-80, (1991) (PubMed).

Fleit, Wright, Unkeless: "Human neutrophil Fc gamma receptor distribution and structure." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 79, Issue 10, pp. 3275-9, (1982) (PubMed).

Guesdon, Ternynck, Avrameas: "The use of avidin-biotin interaction in immunoenzymatic techniques." dans: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 27, Issue 8, pp. 1131-9, (1979) (PubMed).

Détails sur CD16

Antigène: CD16

Autre désignation: CD16

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process