IGLL1 anticorps (Biotin)

N° du produit ABIN2689029

Détail du produit anti-IGLL1 anticorps (Biotin)

Antigène: IGLL1 (Immunoglobulin lambda-Like Polypeptide 1 (IGLL1))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp IGLL1 est conjugé à/à la Biotin

Application: Flow Cytometry (FACS)

Marque: BD Pharmingen™

Attributs du produit: The pre-B cell receptor (pre-BCR) expressed during the early stages of B lymphocyte development is a heterodimer of immunoglobulin heavy chain (IgH) with surrogate light chain, which is an Ig-light-chain-like molecule composed of the non-covalently linked CD179b (λ5) and CD179a (VpreB) proteins. The pre-BCR is believed to control IgH repertoire selection and proliferation of differentiating B lymphocytes. The LM34 antibody reacts with λ5 and surrogate light chain, but not VpreB alone, in transfected X63-Ag8.653 cells. It detects surrogate light chain on pro-B and pre-B cell lines, in the presence or absence of IgH, but not on IgM-positive B lymphocytes. It also detects surrogate light chain or λ5 on the surface and in the cytoplasm of pro-B or pre-B cells from the bone marrow of normal or RAG2-deficient mice. It has been noted that the (CD45R/B220) cell-surface expression of surrogate light chain is upregulated after a one-hour incubation of bone-marrow leukocytes in tissue culture medium at 37 °C. At the earliest stages of B lymphopoiesis, before IgH is available, the surrogate light chain associates with a complex of glycoproteins, including a nonclassical cadherin, which could be involved in selective adhesion events during B-lymphocyte development. After immunization, cell-surface λ5 is detected on a subset of splenic Ig light chain-positive germinal-center B lymphocytes. Two-color analysis of CD179b (?5) expression on bone-marrow pre-B lymphocytes. After immunomagnetic depletion of IgM+ cells using biotinylated anti-mouse IgMb mAb AF6-78 (Cat. no. 553519), C57BL/6 bone-marrow leukocytes were incubated for 1 hour in DMEM at 37 °C to enhance surrogate light chain expression. Then the leukocytes were stained with either biotinylated rat IgG2a, κ isotype control mAb R35-95 (Cat. no. 553928, left panel) or biotinylated mAb LM34 (right panel) in the presence of Mouse BD Fc Block™ purified anti-mouse CD16/CD32 mAb 2.4G2 (Cat. no. 553141/553142), followed by Streptavidin-APC (Cat. no. 554067) and FITC-conjugated anti-mouse CD45R/B220 mAb 9 RA3-6B2 (Cat. no. 553087/553088). Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.

BD Pharmingen™ Biotin Rat Anti-Mouse CD179b - Biotin - Clone LM34 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Purified soluble complex of recombinant mu IgH, lambda5, and V[preB]

Clone: LM34

Isotype: IgG2a kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-IGLL1 (Biotin) (ABIN2689029)

Shimizu, Mundt, Licence, Melchers, Mårtensson: "VpreB1/VpreB2/lambda 5 triple-deficient mice show impaired B cell development but functional allelic exclusion of the IgH locus." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 168, Issue 12, pp. 6286-93, (2002) (PubMed).

Ohnishi, Shimizu, Karasuyama, Melchers: "The identification of a nonclassical cadherin expressed during B cell development and its interaction with surrogate light chain." dans: The Journal of biological chemistry, Vol. 275, Issue 40, pp. 31134-44, (2000) (PubMed).

Mårtensson, Ceredig: "Review article: role of the surrogate light chain and the pre-B-cell receptor in mouse B-cell development." dans: Immunology, Vol. 101, Issue 4, pp. 435-41, (2000) (PubMed).

Melchers, ten Boekel, Seidl, Kong, Yamagami, Onishi, Shimizu, Rolink, Andersson: "Repertoire selection by pre-B-cell receptors and B-cell receptors, and genetic control of B-cell development from immature to mature B cells." dans: Immunological reviews, Vol. 175, pp. 33-46, (2000) (PubMed).

Meffre, Papavasiliou, Cohen, de Bouteiller, Bell, Karasuyama, Schiff, Banchereau, Liu, Nussenzweig: "Antigen receptor engagement turns off the V(D)J recombination machinery in human tonsil B cells." dans: The Journal of experimental medicine, Vol. 188, Issue 4, pp. 765-72, (1998) (PubMed).

Rolink, Grawunder, Winkler, Karasuyama, Melchers: "IL-2 receptor alpha chain (CD25, TAC) expression defines a crucial stage in pre-B cell development." dans: International immunology, Vol. 6, Issue 8, pp. 1257-64, (1995) (PubMed).

Winkler, Rolink, Melchers, Karasuyama: "Precursor B cells of mouse bone marrow express two different complexes with the surrogate light chain on the surface." dans: European journal of immunology, Vol. 25, Issue 2, pp. 446-50, (1995) (PubMed).

Karasuyama, Rolink, Shinkai, Young, Alt, Melchers: "The expression of Vpre-B/lambda 5 surrogate light chain in early bone marrow precursor B cells of normal and B cell-deficient mutant mice." dans: Cell, Vol. 77, Issue 1, pp. 133-43, (1994) (PubMed).

Karasuyama, Rolink, Melchers: "A complex of glycoproteins is associated with VpreB/lambda 5 surrogate light chain on the surface of mu heavy chain-negative early precursor B cell lines." dans: The Journal of experimental medicine, Vol. 178, Issue 2, pp. 469-78, (1993) (PubMed).

Détails sur IGLL1

Antigène: IGLL1 (Immunoglobulin lambda-Like Polypeptide 1 (IGLL1))

Autre désignation: CD179b (IGLL1 Produits)

Synonymes: anticorps 14.1, anticorps AGM2, anticorps CD179b, anticorps IGL1, anticorps IGL5, anticorps IGLJ14.1, anticorps IGLL, anticorps IGO, anticorps IGVPB, anticorps VPREB2, anticorps IGLV, anticorps IGLL1, anticorps MGC151892, anticorps Igll1, anticorps BB139905, anticorps Igl-5, anticorps Igll, anticorps Lambda5, anticorps immunoglobulin lambda like polypeptide 1, anticorps immunoglobulin lambda-like polypeptide 1, anticorps IGLL1, anticorps Igll1

Sujet: Synonyms: λ5

Pathways: Regulation of Cell Size