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CCR7 anticorps

CCR7 Reactivité: Humain FACS Hôte: Souris Monoclonal 2H4 unconjugated
N° du produit ABIN2689060
  • Antigène Voir toutes CCR7 Anticorps
    CCR7 (Chemokine (C-C Motif) Receptor 7 (CCR7))
    Reactivité
    • 99
    • 56
    • 37
    • 16
    • 4
    • 4
    • 2
    • 1
    • 1
    • 1
    Humain
    Hôte
    • 69
    • 29
    • 16
    • 4
    Souris
    Clonalité
    • 62
    • 55
    • 1
    Monoclonal
    Conjugué
    • 50
    • 6
    • 6
    • 5
    • 5
    • 5
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp CCR7 est non-conjugé
    Application
    • 57
    • 57
    • 25
    • 21
    • 18
    • 17
    • 16
    • 15
    • 14
    • 10
    • 6
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS)
    Marque
    BD Pharmingen™
    Attributs du produit
    The monoclonal antibody 2H4 reacts with the human CC chemokine receptor, CCR7/CD197. CCR7 (previously known as BLR-2, EBI-1 and CMKBR7), a seven-transmembrane, G-protein-coupled receptor, is the specific receptor for CC chemokines, MIP-3•/Exodus 3/ELC/CCL19 and 6Ckine/Exodus 2/SLC/TCA4/CCL214. It has been shown that CCR7 mRNA is expressed mainly in lymphoid tissues including spleen, lymph nodes and tonsil. CCR7 mRNA was also detected in peripheral T- and B-lymphocytes, in BM and cord blood CD34-positive cells and mature dendritic cells. The human CCR7 gene, unlike other CC chemokine receptor genes, has been mapped to chromosome 17q12. The immunogen used to generate 2H4 hybridoma was human CCR7 transfected L1.2 mouse lymphoma cells. This antibody is not a neutralizing antibody. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Detection of CCR7 expression on CD4 and CD8-positive human peripheral lymphocytes by purified anti-human CCR7 antibody 2H4 (Cat. No. 550937). Human PBMC were stained with 4.0 μg/10e6 of purified 2H4 using 3-step staining protocol outlined below and anti-human CD45RA-FITC (Cat. No. 555488). The data shown are derived from the CD4- positive (based on staining with anti-human CD4-APC, Cat. No. 555349, left panel) and CD8- positive (based on staining with anti-human CD8-APC, Cat. No. 555369, right panel) lymphocyte gated populations and displayed as bivariate dot plots. Cells were analyzed with a FACScan™ Flow Cytometer

    BD Pharmingen™ Purified Mouse Anti-Human CD197 (CCR7) - Purified - Clone 2H4 - Isotype Mouse IgM, κ - Reactivity Hu - 0.25 mg
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogène
    Human CCR7 transfected L1.2 mouse lymphoma cells
    Clone
    2H4
    Isotype
    IgM kappa
    Top Product
    Discover our top product CCR7 Anticorps primaire
  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Restrictions
    For Research Use only
  • Concentration
    0.5 mg/mL
    Buffer
    Aqueous buffered solution containing ≤0.09 % sodium azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C
    Stockage commentaire
    Store undiluted at 4°C.
  • Sallusto, Lenig, Förster, Lipp, Lanzavecchia: "Two subsets of memory T lymphocytes with distinct homing potentials and effector functions." dans: Nature, Vol. 401, Issue 6754, pp. 708-12, (1999) (PubMed).

    Yoshida, Nagira, Imai, Baba, Takagi, Tabira, Akagi, Nomiyama, Yoshie: "EBI1-ligand chemokine (ELC) attracts a broad spectrum of lymphocytes: activated T cells strongly up-regulate CCR7 and efficiently migrate toward ELC." dans: International immunology, Vol. 10, Issue 7, pp. 901-10, (1998) (PubMed).

    Yanagihara, Komura, Nagafune, Watarai, Yamaguchi: "EBI1/CCR7 is a new member of dendritic cell chemokine receptor that is up-regulated upon maturation." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 6, pp. 3096-102, (1998) (PubMed).

    Yoshida, Nagira, Kitaura, Imagawa, Imai, Yoshie: "Secondary lymphoid-tissue chemokine is a functional ligand for the CC chemokine receptor CCR7." dans: The Journal of biological chemistry, Vol. 273, Issue 12, pp. 7118-22, (1998) (PubMed).

    Yoshida, Imai, Hieshima, Kusuda, Baba, Kitaura, Nishimura, Kakizaki, Nomiyama, Yoshie: "Molecular cloning of a novel human CC chemokine EBI1-ligand chemokine that is a specific functional ligand for EBI1, CCR7." dans: The Journal of biological chemistry, Vol. 272, Issue 21, pp. 13803-9, (1997) (PubMed).

    Burgstahler, Kempkes, Steube, Lipp: "Expression of the chemokine receptor BLR2/EBI1 is specifically transactivated by Epstein-Barr virus nuclear antigen 2." dans: Biochemical and biophysical research communications, Vol. 215, Issue 2, pp. 737-43, (1995) (PubMed).

    Schweickart, Raport, Godiska, Byers, Eddy, Shows, Gray: "Cloning of human and mouse EBI1, a lymphoid-specific G-protein-coupled receptor encoded on human chromosome 17q12-q21.2." dans: Genomics, Vol. 23, Issue 3, pp. 643-50, (1995) (PubMed).

    Birkenbach, Josefsen, Yalamanchili, Lenoir, Kieff: "Epstein-Barr virus-induced genes: first lymphocyte-specific G protein-coupled peptide receptors." dans: Journal of virology, Vol. 67, Issue 4, pp. 2209-20, (1993) (PubMed).

  • Antigène
    CCR7 (Chemokine (C-C Motif) Receptor 7 (CCR7))
    Autre désignation
    CD197 CCR7 (CCR7 Produits)
    Synonymes
    anticorps zgc:165629, anticorps ccr7-a, anticorps CD197, anticorps Cdw197, anticorps Cmkbr7, anticorps EBI1, anticorps Ebi1h, anticorps BLR2, anticorps CDw197, anticorps CMKBR7, anticorps chemokine (C-C motif) receptor 7, anticorps C-C motif chemokine receptor 7, anticorps C-C motif chemokine receptor 7 L homeolog, anticorps ccr7, anticorps CCR7, anticorps ccr7.L, anticorps Ccr7
    Sujet
    Synonyms: BLR-2, EBI-1 and CMKBR7
    Pathways
    Regulation of Actin Filament Polymerization, Positive Regulation of Immune Effector Process
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