CD31 anticorps (Biotin)

N° du produit ABIN2689187

Cet anticorps Souris Monoclonal détecte spécifiquement CD31 dans FACS. Il présente une réactivité avec des échantillons de Souris. Il a été cité dans 5+ publications.

Aperçu rapide pour CD31 anticorps (Biotin) (ABIN2689187)

Antigène: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Reactivité: Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD31 est conjugé à/à la Biotin

Application: Flow Cytometry (FACS)

Clone: TLD

Détail du produit anti-CD31 anticorps (Biotin)

Attributs du produit: The TLD-3A12 antibody reacts with CD31, also known as PECAM-1 (platelet endothelial cell adhesion molecule). CD31 is a ~130 kDa integral membrane glycoprotein, a member of the immunoglobulin super family, that mediates homophilic and heterophilic cell-cell adhesion. CD31 is expressed on endothelial cells, platelets, and subsets of leukocytes. In the human and mouse, multiple alternatively spliced isoforms have been identified, and this alternative splicing may be involved in the regulation of ligand specificity. CD31-mediated endothelial cell-cell interactions are involved in angiogenesis in the mouse and rat. In addition, PECAM-1 has been demonstrated to play an important role in extravasation of leukocytes in vivo, but in vivo treatment with TLD-3A12 mAb had no discernable effect upon the inflammatory infiltrate in experimental allergic encephalomyelitis. The TLD-3A12 mAb partially blocks the proliferation response of antigen-specific CD4+ T cells to antigen-presenting cells and relevant antigen. This mouse anti-rat CD31 antibody cross-reacts with pig endothelial cells and platelets, as determined by immunohistochemical staining of acetone-fixed frozen sections and immunofluorescent staining with flow cytometric analysis, respectively. A suspension of Lewis rat microglial cells were used as the source of the immunogen. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. The expression of CD31 on rat spleen leukocytes. LEW splenocytes were simultaneously stained with biotinylated mAb TLD-3A12 (right panel) and FITC-conjugated anti-rat CD45RA mAb OX-33 (Cat. No. 554883), followed by Streptavidin-PE (Cat. No. 554061). Flow cytometry was performed on a BD FACScan™ flow cytometry system.

BD Pharmingen™ Biotin Mouse Anti-Rat CD31 - Biotin - Clone TLD-3A12 - Isotype Mouse IgG1, κ - 0.5 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Lewis Rat Microglia

Isotype: IgG1 kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Références pour anticorps anti-CD31 (Biotin) (ABIN2689187)

DeLisser, Christofidou-Solomidou, Strieter, Burdick, Robinson, Wexler, Kerr, Garlanda, Merwin, Madri, Albelda: "Involvement of endothelial PECAM-1/CD31 in angiogenesis." dans: The American journal of pathology, Vol. 151, Issue 3, pp. 671-7, (1997) (PubMed).

Famiglietti, Sun, DeLisser, Albelda: "Tyrosine residue in exon 14 of the cytoplasmic domain of platelet endothelial cell adhesion molecule-1 (PECAM-1/CD31) regulates ligand binding specificity." dans: The Journal of cell biology, Vol. 138, Issue 6, pp. 1425-35, (1997) (PubMed).

Williams, Zhao, Ueno, Hickey: "PECAM-1 (CD31) expression in the central nervous system and its role in experimental allergic encephalomyelitis in the rat." dans: Journal of neuroscience research, Vol. 45, Issue 6, pp. 747-57, (1997) (PubMed).

Wakelin, Sanz, Dewar, Albelda, Larkin, Boughton-Smith, Williams, Nourshargh: "An anti-platelet-endothelial cell adhesion molecule-1 antibody inhibits leukocyte extravasation from mesenteric microvessels in vivo by blocking the passage through the basement membrane." dans: The Journal of experimental medicine, Vol. 184, Issue 1, pp. 229-39, (1996) (PubMed).

DeLisser, Newman, Albelda: "Molecular and functional aspects of PECAM-1/CD31." dans: Immunology today, Vol. 15, Issue 10, pp. 490-5, (1994) (PubMed).

Détails sur CD31

Antigène: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Autre désignation: CD31

Sujet: Synonyms: PECAM-1

Pathways: Regulation of Actin Filament Polymerization