CD40 anticorps

N° du produit ABIN2689246

Détail du produit anti-CD40 anticorps

Antigène: CD40

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp CD40 est non-conjugé

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc))

Marque: BD Pharmingen™

Attributs du produit: The 3/23 clone reacts with CD40, a 40-50 kDa glycoprotein expressed on B lymphocytes and other antigen-presenting cells. CD40 has been reported to be transiently expressed on activated CD4+ and CD8+ T cells and in some mouse strains, the 3/23 mAb has been reported to react with 5-10 % of T lymphocytes in adult mouse, but not neonatal, spleen. CD40 plays a key role in B-cell growth and differentiation where interactions of CD40 with its ligand, CD154, are involved in the initiation, effector, and memory stages of cell-mediated immune responses. In addition, CD40 has been reported to be involved with the triggering of NK cells and NK-T cells. Ligation of CD40 with the 3/23 antibody has been reported to induce splenic B cells to express the costimulatory molecule CD86 (B7-2). In addition, although the 3/23 antibody by itself is a weak B-cell mitogen, it has been reported to synergize markedly with mitogenic anti-IgM, anti-IgD mAb or IL-4 to promote B-cell proliferation. Immunohistochemical staining of CD40. Frozen sections of normal mouse thymus was reacted with the 3/23 antibody. B lymphocytes and dendritic cells can be identified by the brown labeling of their cell surface membranes. Amplification 20x.

BD Pharmingen™ Purified Rat Anti-Mouse CD40 - Purified - Clone 3/23 - Isotype Rat IgG2a, κ - Reactivity Ms - 1 mL

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Mouse CD40 Recombinant Protein

Clone: 3-23

Isotype: IgG2a kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 125 μg/mL

Buffer: Aqueous buffered solution containing BSA, goat serum, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-CD40 (ABIN2689246)

Klaus, Holman, Hasbold: "Properties of mouse CD40: the role of homotypic adhesion in the activation of B cells via CD40." dans: European journal of immunology, Vol. 24, Issue 11, pp. 2714-9, (1994) (PubMed).

Parry, Hasbold, Holman, Klaus: "Hypercross-linking surface IgM or IgD receptors on mature B cells induces apoptosis that is reversed by costimulation with IL-4 and anti-CD40." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 6, pp. 2821-9, (1994) (PubMed).

Hasbold, Johnson-Léger, Atkins, Clark, Klaus: "Properties of mouse CD40: cellular distribution of CD40 and B cell activation by monoclonal anti-mouse CD40 antibodies." dans: European journal of immunology, Vol. 24, Issue 8, pp. 1835-42, (1994) (PubMed).

Hasbold, Klaus: "B cells from CBA/N mice do not proliferate following ligation of CD40." dans: European journal of immunology, Vol. 24, Issue 1, pp. 152-7, (1994) (PubMed).

Noelle, Ledbetter, Aruffo: "CD40 and its ligand, an essential ligand-receptor pair for thymus-dependent B-cell activation." dans: Immunology today, Vol. 13, Issue 11, pp. 431-3, (1993) (PubMed).

Détails sur CD40

Antigène: CD40

Autre désignation: CD40 (CD40 Produits)

Synonymes: anticorps Bp50, anticorps CDW40, anticorps TNFRSF5, anticorps p50, anticorps AI326936, anticorps GP39, anticorps HIGM1, anticorps IGM, anticorps IMD3, anticorps T-BAM, anticorps TRAP, anticorps Tnfrsf5, anticorps TNFSF5, anticorps CD40 molecule, anticorps CD40 antigen, anticorps CD40, anticorps Cd40

Pathways: Signalisation NF-kappaB, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints