CD55 anticorps (Biotin)

N° du produit ABIN2689338

Il existe 3+ publications pour ce produit. L’anticorps anti-CD55 Monoclonal Souris est utilisé pour la détection de CD55 dans des échantillons de Humain. Il a été validé pour FACS.

Aperçu rapide pour CD55 anticorps (Biotin) (ABIN2689338)

Antigène: CD55 (Complement Decay-Accelerating Factor (CD55))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD55 est conjugé à/à la Biotin

Application: Flow Cytometry (FACS)

Clone: IA10

Détail du produit anti-CD55 anticorps (Biotin)

Attributs du produit: Reacts with CD55, decay-accelerating factor (DAF), a glycosylphosphatidylinositol (GPI)-anchored single chain glycoprotein of approximately 70 kDa, expressed on hematopoietic cells. It has been suggested that the role of DAF is to protect cells from damage by autologous complement preventing the amplification steps of the complement cascade by interfering with the assembly of the C3-convertases, C4b2a and C3bBb, and the C5-convertases, C4b2a3b and C3bBb3b. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Profile of K562 cells expressing glycosylphosphatidylinositol (GPI) anchored protein analyzed on a FACScan Profile of glycosylphosphatidylinositol (GPI) achor-defective mutant K562 cell line lymphocytes analyzed on a FACScan

BD Pharmingen™ Biotin Mouse Anti-Human CD55 - Biotin - Clone IA10 - Isotype Mouse IgG2a, κ - Reactivity Hu - 100 Tests

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Isotype: IgG2a kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Volume d'échantillon: 20 μL

Restrictions: For Research Use only

Stockage

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Références pour anticorps anti-CD55 (Biotin) (ABIN2689338)

Loveland, Szokolai, Johnstone, McKenzie: "Coordinate functions of multiple complement regulating molecules, CD46, CD55, and CD59." dans: Transplantation proceedings, Vol. 26, Issue 3, pp. 1070-1, (1994) (PubMed).

Kinoshita, Medof, Silber, Nussenzweig: "Distribution of decay-accelerating factor in the peripheral blood of normal individuals and patients with paroxysmal nocturnal hemoglobinuria." dans: The Journal of experimental medicine, Vol. 162, Issue 1, pp. 75-92, (1985) (PubMed).

Guesdon, Ternynck, Avrameas: "The use of avidin-biotin interaction in immunoenzymatic techniques." dans: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 27, Issue 8, pp. 1131-9, (1979) (PubMed).

Détails sur CD55

Antigène: CD55 (Complement Decay-Accelerating Factor (CD55))

Autre désignation: CD55

Sujet: Synonyms: DAF

Pathways: Système du Complément, Regulation of Leukocyte Mediated Immunity