Cutaneous Lymphocyte Antigen anticorps (Biotin)

N° du produit ABIN2689480

Cité dans 5 publications. Cet anticorps anti-Cutaneous Lymphocyte Antigen Monoclonal Rat conjugué à Biotin (Clone HECA) (ABIN2689480) détecte spécifiquement Cutaneous Lymphocyte Antigen dans FACS, IHC (f) et IHC (fro). L’anticorps est réactif avec des échantillons de Humain et Souris.

Aperçu rapide pour Cutaneous Lymphocyte Antigen anticorps (Biotin) (ABIN2689480)

Antigène: Cutaneous Lymphocyte Antigen

Reactivité: Humain, Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Biotin

Application: Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: HECA

Détail du produit

Attributs du produit: Reacts with cutaneous lymphocyte associated antigen (CLA), a carbohydrate domain shared by sialyl Lewis[x] (sLe[x]) and sialyl Lewis[a] (sLe[a]) antigens. It serves as the ligand for selectins including CD62E (ELAM-1) and CD62L (LECAM-1). CLA is expressed on lymphocytes in the skin on high endothelium. In peripheral blood, it is expressed on a small subset of lympocytes and all monocytes and granulocytes. It has been suggested that CLA plays a role in supporting lymphocyte migration to extravascular tissue during inflammation. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Profile of peripheral blood lymphocytes analyzed by flow cytometry

BD Pharmingen™ Biotin Rat Anti-Human Cutaneous Lymphocyte Antigen - Biotin - Clone HECA-452 - Isotype Rat IgM, κ - Reactivity Hu, Ms - 100 Tests

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Isotype: IgM kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Volume d'échantillon: 20 μL

Restrictions: For Research Use only

Stockage

Buffer: Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Référence

Borges, Pendl, Eytner, Steegmaier, Zöllner, Vestweber: "The binding of T cell-expressed P-selectin glycoprotein ligand-1 to E- and P-selectin is differentially regulated." dans: The Journal of biological chemistry, Vol. 272, Issue 45, pp. 28786-92, (1997) (PubMed).

Picker, Kishimoto, Smith, Warnock, Butcher: "ELAM-1 is an adhesion molecule for skin-homing T cells." dans: Nature, Vol. 349, Issue 6312, pp. 796-9, (1991) (PubMed).

Picker, Warnock, Burns, Doerschuk, Berg, Butcher: "The neutrophil selectin LECAM-1 presents carbohydrate ligands to the vascular selectins ELAM-1 and GMP-140." dans: Cell, Vol. 66, Issue 5, pp. 921-33, (1991) (PubMed).

Duijvestijn, Horst, Pals, Rouse, Steere, Picker, Meijer, Butcher: "High endothelial differentiation in human lymphoid and inflammatory tissues defined by monoclonal antibody HECA-452." dans: The American journal of pathology, Vol. 130, Issue 1, pp. 147-55, (1988) (PubMed).

Guesdon, Ternynck, Avrameas: "The use of avidin-biotin interaction in immunoenzymatic techniques." dans: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 27, Issue 8, pp. 1131-9, (1979) (PubMed).

Détail du antigène

Antigène: Cutaneous Lymphocyte Antigen

Sujet: Synonyms: CLA