CXCR5 anticorps (Biotin)

N° du produit ABIN2689484

Cet anticorps Rat Monoclonal détecte spécifiquement CXCR5 dans FACS. Il présente une réactivité envers Souris et a été mentionné dans 1 publication.

Aperçu rapide pour CXCR5 anticorps (Biotin) (ABIN2689484)

Antigène: CXCR5 (Chemokine (C-X-C Motif) Receptor 5 (CXCR5))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp CXCR5 est conjugé à/à la Biotin

Application: Flow Cytometry (FACS)

Clone: 2G8

Détail du produit anti-CXCR5 anticorps (Biotin)

Marque: BD Pharmingen™

Attributs du produit: The monoclonal antibody 2G8 reacts with the mouse CXC chemokine receptor, CXCR5. CXCR5 (a.k.a. BLR1, NLR and MDR15), a seven-transmembrane, G-protein-coupled receptor, is the specific receptor for CXC chemokines, CXCL13/BLC/BCA-1. The expression of CXCR5 has been detected in spleen, lymph nodes, tonsils, brain, bone marrow, T cells, B cells, cerebrum, cerebellum, hippcampus and pituitary. In mouse spleen, CXCR5 was strictly expressed by mature B cells and a small subset of T lymphocytes. The immunogen used to generate 2G8 hybridoma was a recombinant protein containing N-terminal amino acids of mouse CXCR5 (GST-NmBLR1). This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature. Detection of CXCR5 expression on mouse splenocytes by biotinylated 2G8. Mouse splenocytes were stained with 1.0 μg/test of biotinylated 2G8, Streptavidin-PE (Cat. No. 554061) and anti-mouse CD45R/B220-FITC (Cat. No. 553088). The data reflects gating on lymphocytes, based on forward and side scattered light signals. The level of nonspecific staining was assessed by using biotinylated rat IgG2a (cat. No. 553928) as isotype control. The quadrant markers for the bivariate dot plots were set based on the isotype control.

BD Pharmingen™ Biotin Rat Anti-Mouse CXCR5 - Biotin - Clone 2G8 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.1 mg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Mouse CXCR5

Isotype: IgG2a kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Références pour anticorps anti-CXCR5 (Biotin) (ABIN2689484)

Förster, Mattis, Kremmer, Wolf, Brem, Lipp: "A putative chemokine receptor, BLR1, directs B cell migration to defined lymphoid organs and specific anatomic compartments of the spleen." dans: Cell, Vol. 87, Issue 6, pp. 1037-47, (1997) (PubMed).

Détails sur CXCR5

Antigène: CXCR5 (Chemokine (C-X-C Motif) Receptor 5 (CXCR5))

Autre désignation: CXCR5