FOXP3 anticorps
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- Antigène Voir toutes FOXP3 Anticorps
- FOXP3 (Forkhead Box P3 (FOXP3))
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Reactivité
- Humain, Cynomolgus, Macaque rhésus, Babouin
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Hôte
- Souris
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Clonalité
- Monoclonal
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Conjugué
- Cet anticorp FOXP3 est non-conjugé
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Intracellular Staining (ICS)
- Marque
- BD Pharmingen™
- Attributs du produit
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The 259D/C7 antibody reacts with the human FoxP3 transcription factor, a member of the forkhead or winged helix family of transcription factors. The expression of FoxP3, also known as Scurfin, IPEX and JM2, has been found to be associated with CD4+ regulatory T cells and represents a specific marker for these cells. Flow-cytometric analysis has shown that FoxP3 is expressed by the majority of CD4+CD25+high T cells in peripheral blood while less than half of CD4+CD25int cell population are FoxP3 positive. Approximately 5-10 % of peripheral CD4+ cells are CD4+CD25+ T regulatory cells. T regulatory cells are thought to play a critical role in the control of T cell mediated autoimmunity by suppressing the proliferation and cytokine production of other T cells. To support this hypothesis, it has been found that FOXP3 is mutated in scurfy (sf) mice. The 259D/C7 antibody reacts with all currently identified isoforms of human FoxP3 and is cross-reactive with Cynomolgus, Rhesus and Baboon. Flow cytometric analysis of Purified anti-Human FoxP3 on resting PBMC. Human PBMC were fixed and permeabilized using working solutions of FoxP3 Buffers A and C (Cat. No. 560098. see Recommended Assay Procedure), Intracellular staining with Purified Mouse anti-Human FoxP3 (clone 259D/C7, Cat. No. 560044) was followed by secondary staining with APC Rat anti-Mouse IgG1 (
BD Pharmingen™ Purified Mouse anti-Human FoxP3 - Purified - Clone 259D/C7 - Isotype Mouse IgG1 - Reactivity Hu, Bab, Cyno, Rhe - 0.1 mg - Purification
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- Immunogène
- FoxP3 recombinant protein
- Clone
- 259D-C7
- Isotype
- IgG1
- Top Product
- Discover our top product FOXP3 Anticorps primaire
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- Indications d'application
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Concentration
- 0.5 mg/mL
- Buffer
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C
- Stockage commentaire
- Store undiluted at 4°C.
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Analysis of FOXP3 protein expression in human CD4+CD25+ regulatory T cells at the single-cell level." dans: European journal of immunology, Vol. 35, Issue 6, pp. 1681-91, (2005) (PubMed).
: "Disruption of a new forkhead/winged-helix protein, scurfin, results in the fatal lymphoproliferative disorder of the scurfy mouse." dans: Nature genetics, Vol. 27, Issue 1, pp. 68-73, (2001) (PubMed).
: "X-linked neonatal diabetes mellitus, enteropathy and endocrinopathy syndrome is the human equivalent of mouse scurfy." dans: Nature genetics, Vol. 27, Issue 1, pp. 18-20, (2001) (PubMed).
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Analysis of FOXP3 protein expression in human CD4+CD25+ regulatory T cells at the single-cell level." dans: European journal of immunology, Vol. 35, Issue 6, pp. 1681-91, (2005) (PubMed).
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- Antigène
- FOXP3 (Forkhead Box P3 (FOXP3))
- Autre désignation
- FoxP3 (FOXP3 Produits)
- Synonymes
- anticorps foxp3, anticorps FOXP3, anticorps AIID, anticorps DIETER, anticorps IPEX, anticorps PIDX, anticorps XPID, anticorps JM2, anticorps scurfin, anticorps sf, anticorps RGD1562112, anticorps forkhead box P3, anticorps forkhead box P3a, anticorps forkhead box P3 L homeolog, anticorps FOXP3, anticorps foxp3a, anticorps foxp3, anticorps foxp3.L, anticorps Foxp3
- Sujet
- Synonyms: Scurfin, IPEX, JM2
- Pathways
- Chromatin Binding, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation
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