The MQ1-17H12 antibody reacts with human interleukin-2 (IL-2). The immunogen used to generate the MQ1-17H12 hybridoma was recombinant human IL-2. Unconjugated or purified forms of this antibody have been reported to be neutralizing for human IL-2 bioactivity. Expression of IL-2 by stimulated CD3+ human PBMC. Human PBMCs were stimulated for 6 hours with PMA (50 ng/mL final concentration, Sigma, Cat. #P-8139) and calcium ionophore A23187 (500-100 ng/mL final concentration, Sigma, Cat. #C-9275) in the presence of BD GolgiStop™ (2 μM final concentration, Cat. No. 554724). The PBMCs were stained with PE-Cy™5-anti-CD3 (PE-Cy™5-UCHT1, Cat. No. 555334), fixed, permeabilized, and then stained with 0.25 μg of FITC-rat anti-human IL-2 antibody (FITC-MQ1-17H12, Cat. No. 554565) using Pharmingen's staining protocol (left panel). To demonstrate specificity of staining, the binding of FITC-MQ1-17H12 was blocked by the preincubation of the fluorochrome - conjugated antibody with recombinant human IL-2 (10 ng/mL final concentration, Cat. No. 554603, middle panel), and by preincubation of the fixed/permeabilized cells with unlabeled MQ1-17H12 antibody (5.0 μg, Cat. No. 554563, right panel) prior to staining with the FITC-MQ1-17H12 antibody. The quadrant markers for the bivariate dot plots were set based on the autofluorescence control, and verified with the recombinant cytokine blocking (middle panel) and unlabeled antibody blocking (right panel) specificity controls.
BD Pharmingen™ Purified Rat Anti-Human IL-2 - Purified - Clone MQ1-17H12 - Isotype Rat IgG2a, κ - Reactivity Hu - 0.1 mg
Purification
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C
Stockage commentaire
Store undiluted at 4°C.
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