NKX6-1 anticorps

N° du produit ABIN2689864

Détail du produit anti-NKX6-1 anticorps

Antigène: NKX6-1 (NK6 Homeobox 1 (NKX6-1))

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp NKX6-1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), BioImaging (BI), Intracellular Staining (ICS)

Marque: BD Pharmingen™

Attributs du produit: The R11-560 monoclonal antibody specifically binds to human and mouse homeobox protein Nkx6.1. The transcription factor Nkx6.1 plays a role in pancreatic islet beta cell development through the regulation of HNF1a and insulin genes. Nkx6.1 is considered to be a highly specific transcription factor marking pancreatic beta islet cells, where it is expressed in both developing and mature cells. In addition, Nkx6.1 functions as a transcriptional repressor induced by the Sonic Hedgehog (Shh) signaling pathway in fate determination of several neuronal cell types. During antibody development, the purified R11-560 monoclonal antibody was found to detect Nkx6.1 by western blot and indirect immunofluorescent staining followed by flow cytometric analysis or imaging analysis. Western Blot analysis of Nkx6.1 in mouse pancreatic tumor (insulinoma) cell lysate. Lysate from Beta-T-C6 cells (ATCC, CRL-11506™) was probed with Purified Mouse Anti-Nkx6.1 monoclonal antibody at titrations of 0.5 (lane 1), 0.25 (lane 2), and 0.125 μg/mL (lane 3). Nkx6.1 is identified as a doublet of approximately 44 kDa. Immunoflourescent staining of Nkx6.1 in mouse pancreatic tumor (insulinoma) cells. Beta-T-C6 cells (ATCC, CRL-11506™) were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655), permeabilized with BD Phosflow™ Perm III Buffer (Cat. No. 558050, and stained with Purified Mouse Anti-Nkx6.1 monoclonal antibody (pseudo-colored green) at 1.2 μg/mL. The second-step reagent was Alexa Fluor® 488 goat anti-mouse Ig (Life Technologies), and counter-staining was with DAPI (pseudo-colored blue). The images were captured on a BD Pathway™ 435 Cell Analyzer and merged using BD AttoVision™ Software.

BD Pharmingen™ Purified Mouse Anti-Nkx6.1 - Purified - Clone R11-560 - Isotype Mouse IgG1, κ - Reactivity Ms, Hu - 100 μg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human Nkx6.1 Recombinant Protein

Clone: R11

Isotype: IgG1 kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-NKX6-1 (ABIN2689864)

Donelan, Koya, Li, Yang: "Distinct regulation of hepatic nuclear factor 1alpha by NKX6.1 in pancreatic beta cells." dans: The Journal of biological chemistry, Vol. 285, Issue 16, pp. 12181-9, (2010) (PubMed).

Prakash, Puelles, Freude, Trümbach, Omodei, Di Salvio, Sussel, Ericson, Sander, Simeone, Wurst: "Nkx6-1 controls the identity and fate of red nucleus and oculomotor neurons in the mouse midbrain." dans: Development (Cambridge, England), Vol. 136, Issue 15, pp. 2545-55, (2009) (PubMed).

Nelson, Schaffer, Sander: "The transcription factors Nkx6.1 and Nkx6.2 possess equivalent activities in promoting beta-cell fate specification in Pdx1+ pancreatic progenitor cells." dans: Development (Cambridge, England), Vol. 134, Issue 13, pp. 2491-500, (2007) (PubMed).

Pedersen, Klinck, Hecksher-Sorensen, Zahn, Madsen, Serup, Jorgensen: "Generation and characterization of monoclonal antibodies against the transcription factor Nkx6.1." dans: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 54, Issue 5, pp. 567-74, (2006) (PubMed).

DAmour, Bang, Eliazer, Kelly, Agulnick, Smart, Moorman, Kroon, Carpenter, Baetge: "Production of pancreatic hormone-expressing endocrine cells from human embryonic stem cells." dans: Nature biotechnology, Vol. 24, Issue 11, pp. 1392-401, (2006) (PubMed).

Inoue, Rudnick, German, Veile, Donis-Keller, Permutt: "Isolation, characterization, and chromosomal mapping of the human Nkx6.1 gene (NKX6A), a new pancreatic islet homeobox gene." dans: Genomics, Vol. 40, Issue 2, pp. 367-70, (1997) (PubMed).

Détails sur NKX6-1

Antigène: NKX6-1 (NK6 Homeobox 1 (NKX6-1))

Autre désignation: Nkx6.1 (NKX6-1 Produits)

Synonymes: anticorps nkx6a, anticorps nkx6.1, anticorps NKX6.1, anticorps NKX6A, anticorps zgc:92879, anticorps Nkx6.1, anticorps Nkx61, anticorps Nkx6a, anticorps NK6 homeobox 1, anticorps NK6 homeobox 1 L homeolog, anticorps nkx6-1, anticorps NKX6-1, anticorps nkx6.1, anticorps nkx6-1.L, anticorps Nkx6-1

Sujet: Synonyms: Nkx6.1, NKX6-1, NKX6A, Nkx6-1, Nkx6.1, Nkx6a

Poids moléculaire: ~44 kDa

Pathways: Dopaminergic Neurogenesis, Positive Regulation of Peptide Hormone Secretion