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SIGLEC5 anticorps

SIGLEC5 Reactivité: Souris ELISA, FACS, IHC (fro), IF Hôte: Rat Monoclonal E50 unconjugated
N° du produit ABIN2689902
  • Antigène Voir toutes SIGLEC5 Anticorps
    SIGLEC5 (Sialic Acid Binding Ig-Like Lectin 5 (SIGLEC5))
    Reactivité
    • 67
    • 28
    • 4
    Souris
    Hôte
    • 59
    • 8
    • 3
    Rat
    Clonalité
    • 59
    • 11
    Monoclonal
    Conjugué
    • 26
    • 11
    • 8
    • 8
    • 6
    • 5
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp SIGLEC5 est non-conjugé
    Application
    • 55
    • 36
    • 16
    • 7
    • 6
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    ELISA, Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF)
    Marque
    BD Pharmingen™
    Attributs du produit
    The E50-2440 antibody reacts with Siglec-F, the sixth siglec protein to be reported in the mouse. Siglecs are the sialic acid-binding immunoglobulin superfamily lectins defined in the human, each of which has a distinctive expression pattern in the hematopoietic system and at least some of which are known to mediate cell-cell interactions. Orthologous proteins of human Siglec-1 (Sialoadhesin or CD169), Siglec-2 (CD22), and Siglec-4 (myelin-associated glycoprotein) have been characterized in the mouse. Human Siglec-3 (CD33) and Siglecs-5 through -10 are encoded by a cluster of closely related genes, and each has two cytoplasmic ITIM (Immunoreceptor Tyrosine-based Inhibitory Motifs). Similarly, mouse Siglec-F is encoded by a gene in a syntenic cluster in the mouse, and the protein has sialic acid-binding activity and an intracytoplasmic ITIM. Its expression pattern differs from those of the human Siglec-3-related proteins in that it is found on immature cells of the myelomonocytic lineage, with reduced expression on mature neutrophils and monocytes, and not on lymphoid cells. It has been proposed that mAb E50-2440 may be used for identification of immature myelomonocytic cells in the mouse. Two-color analysis of Siglec-F expression on bone-marrow myeloid cells. BALB/c bone-marrow leukocytes were stained with either purified rat IgG2a, κ isotype control mAb R35-95 (Cat. No. 553927, left panel) or purified mAb E50-2440 (right panel) in the presence of Mouse Fc Block™ (purified anti-mouse CD16/CD32 mAb 2.4G2, Cat. No. 553141/553142), followed by biotinylated anti-rat IgG2a mAb RG7/1.30 (Cat. No. 553894), then Streptavidin-PE (Cat. No. 554061) and FITC-conjugated anti-mouse CD11b (Integrin αM chain) mAb M1/70 (Cat. No. 557396/553310). Flow cytometry was performed on a FACSCalibur™ .

    BD Pharmingen™ Purified Rat Anti-Mouse Siglec-F - Purified - Clone E50-2440 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.1 mg
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogène
    Mouse Siglec-F and human IgG Fc recombinant fusion protein
    Clone
    E50
    Isotype
    IgG2a kappa
    Top Product
    Discover our top product SIGLEC5 Anticorps primaire
  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Restrictions
    For Research Use only
  • Concentration
    0.5 mg/mL
    Buffer
    Aqueous buffered solution containing ≤0.09 % sodium azide.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    4 °C
    Stockage commentaire
    Store undiluted at 4°C.
  • Angata, Hingorani, Varki, Varki: "Cloning and characterization of a novel mouse Siglec, mSiglec-F: differential evolution of the mouse and human (CD33) Siglec-3-related gene clusters." dans: The Journal of biological chemistry, Vol. 276, Issue 48, pp. 45128-36, (2001) (PubMed).

    Crocker, Varki: "Siglecs, sialic acids and innate immunity." dans: Trends in immunology, Vol. 22, Issue 6, pp. 337-42, (2001) (PubMed).

  • Antigène
    SIGLEC5 (Sialic Acid Binding Ig-Like Lectin 5 (SIGLEC5))
    Autre désignation
    Siglec-F (SIGLEC5 Produits)
    Synonymes
    anticorps CD170, anticorps CD33L2, anticorps OB-BP2, anticorps OBBP2, anticorps SIGLEC-5, anticorps sialic acid binding Ig like lectin 5, anticorps sialic acid-binding Ig-like lectin 5, anticorps sialic acid binding Ig-like lectin 5, anticorps SIGLEC5, anticorps LOC100597560, anticorps Siglec5
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