CD147 anticorps

N° du produit ABIN2689942

Détail du produit anti-CD147 anticorps

Antigène: CD147 (BSG) (Basigin (Ok Blood Group) (BSG))

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD147 est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), BioImaging (BI)

Marque: BD Pharmingen™

Attributs du produit: The TRA-1-85 monoclonal antibody (mAb) specifically binds to the high frequency blood group antigen OKa. The OKa blood group antigen is a 35-69 kDa glycoprotein also known as CD147, EMMPRIN, M6, or Basigin. The OKa group is widely expressed on most human tissues including, but not limited to kidney, peripheral blood leukocytes, liver, pancreas, colon, skin, and brain. TRA-1-85 mAb is often used to discern human cells from non-human cells in multiple applications including animal transplantation studies. During development, the purified TRA-1-85 mAb was found to detect OKa blood group antigen by western blot analysis of cellular lysates, by immunofluorescent staining of fixed cells, and by flow cytometric analysis of live cells. Flow cytometric analysis of TRA-1-85 expression on human cervical carcinoma (HeLa cell line). HeLa cells (ATCC® CCL 2™) were stained with Purified Mouse anti-Human TRA-1-85 Antigen monoclonal antibody (solid line histogram) or with Purified Mouse IgG1, κ Isotype Control (Cat. No. 554121, dashed line histogram). The second-step reagent was FITC goat anti-Mouse Ig (Cat. No. 554001). The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of the HeLa cell line. Flow cytometry was performed using a BD LSRFortessa™ Flow Cytometer System. Immunoflourescent staining of Tra-1-85 on human embryonic stem (ES) cells. H9 human ES cells (WiCell, Madison, WI) passage 47 grown in mTESR™1 media (StemCell Technologies) on BD Matrigel™ hESC-qualified Matrix (Cat. No. 354277) were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655). The cells were stained with Purified Mouse anti-Human TRA-1-85 Antigen monoclonal antibody (pseudo-colored green) at 1.2 μg/mL. The second-step reagent was Alexa Fluor® 488 goat anti-mouse Ig (Life Technologies), and cell nuclei were stained with Hoechst (pseudo-colored blue). The images were captured on a BD Pathway™ 435 Cell Analyzer and merged using BD Attovision™ software.

BD Pharmingen™ Purified Mouse anti-Human TRA-1-85 Antigen - Purified - Clone TRA-1-85 - Isotype Mouse IgG1, κ - Reactivity Hu, Ms - 100 μg

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human Embryonal Carcinoma Cell Line

Clone: TRA-1-85

Isotype: IgG1 kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 4°C.

Références pour anticorps anti-CD147 (ABIN2689942)

Choi, Yu, Smuga-Otto, Salvagiotto, Rehrauer, Vodyanik, Thomson, Slukvin: "Hematopoietic and endothelial differentiation of human induced pluripotent stem cells." dans: Stem cells (Dayton, Ohio), Vol. 27, Issue 3, pp. 559-67, (2009) (PubMed).

Olivier, Rybicki, Bouhassira: "Differentiation of human embryonic stem cells into bipotent mesenchymal stem cells." dans: Stem cells (Dayton, Ohio), Vol. 24, Issue 8, pp. 1914-22, (2006) (PubMed).

Draper, Pigott, Thomson, Andrews: "Surface antigens of human embryonic stem cells: changes upon differentiation in culture." dans: Journal of anatomy, Vol. 200, Issue Pt 3, pp. 249-58, (2002) (PubMed).

Williams, Daniels, Pym, Sheer, Povey, Okubo, Andrews, Goodfellow: "Biochemical and genetic analysis of the OKa blood group antigen." dans: Immunogenetics, Vol. 27, Issue 5, pp. 322-9, (1988) (PubMed).

Détails sur CD147

Antigène: CD147 (BSG) (Basigin (Ok Blood Group) (BSG))

Autre désignation: CD147 (BSG Produits)

Synonymes: anticorps EMMPRIN, anticorps RPE7, anticorps 5F7, anticorps CD147, anticorps M6, anticorps OK, anticorps TCSF, anticorps AI115436, anticorps AI325119, anticorps HT-7, anticorps 5A11, anticorps CE9, anticorps HT7, anticorps Ox47R, anticorps basignin, anticorps wu:fb26a05, anticorps wu:fo98e12, anticorps bsg, anticorps prso03, anticorps basigin (Ok blood group), anticorps basigin, anticorps basigin (Ok blood group) S homeolog, anticorps BSG, anticorps Bsg, anticorps bsg, anticorps bsg.S

Sujet: Synonyms: OKa, CD147, EMMPRIN, Basigin

Pathways: S100 Proteins