Fc gamma RII (CD32) anticorps

N° du produit ABIN7304806

Cet anticorps Souris Monoclonal détecte spécifiquement Fc gamma RII dans FACS et IF. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour Fc gamma RII (CD32) anticorps (ABIN7304806)

Antigène: Fc gamma RII (CD32)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: IV-3

Détail du produit

Fonction: Mouse monoclonal antibody to CD32

Specificité: Recognizes human CD32

Attributs du produit: Mouse monoclonal antibody to CD32

Purification: The antibody was purified by affinity chromatography.

Immunogène: Native purified human CD32.

Isotype: IgG2a

Information d'application

Indications d'application: IF/IC (1/50 - 1/200), FC (1/500 - 1/2000)

Protocole: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Date de péremption: 12 months

Détail du antigène

Antigène: Fc gamma RII (CD32)

Autre désignation: CD32

Sujet: T3D, T-cell surface glycoprotein CD3 delta chain, T-cell receptor T3 delta chain, CD antigen CD3d, T3E, T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD antigen CD3e, T3G, T-cell surface glycoprotein CD3 gamma chain, T-cell receptor T3 gamma chain, CD antigen CD3g, CD3Z, T3Z, TCRZ, T-cell surface glycoprotein CD3 zeta chain, T-cell receptor T3 zeta chain, CD antigen CD247

ID gène: 2212

UniProt: P12318