NCK2 anticorps

N° du produit ABIN271941

Cet anticorps anti-NCK2 Polyclonal Lapin (ABIN271941) détecte spécifiquement NCK2 dans IHC (p). L’anticorps est réactif avec des échantillons de Humain et Souris.

Aperçu rapide pour NCK2 anticorps (ABIN271941)

Antigène: NCK2 (NCK Adaptor Protein 2 (NCK2))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NCK2 est non-conjugé

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-NCK2 anticorps

Specificité: This antibody detects endogenous levels of NCK2 protein. (region surrounding Ile364)

Réactivité croisée (Details): Species reactivity (expected):Mouse.
Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Pureté: > 95 % pure by SDS-PAGE

Information d'application

Indications d'application: ELISA: 1: 1000approx. 1: 5000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: DO NOT FREEZE!

Stock: 4 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C.

Détails sur NCK2

Antigène: NCK2 (NCK Adaptor Protein 2 (NCK2))

Autre désignation: NCK2

Sujet: The NCK family of SH2/SH3 adaptor proteins consists of two members, NCK1 (NCKα) and NCK2 (NCKβ), which couple tyrosine kinase signaling, including the EGF and PDGF receptor-pathways, to downstream signaling proteins. Specifically, overexpression of NCK1 in NIH/3T3 cells decreases DNA synthesis stimulated by EGF. Furthermore, the SH2 domain of NCK2 inhibits EGF- and PDGF-induced DNA synthesis. The SH3 domain of NCK binds a proline-rich domain on PAK, a known actin cytoskeleton regulator. The NCK protein thus mediates the interaction between PAK and RAC. The NCK2 protein binds human PDGFR-b (Tyr-1009), overexpression of NCK2 inhibits PDGF-induced membrane ruffling and lamellipod formation. Various growth factor receptors, cell surface antigens and adhesion molecules phosphorylate mammalian NCK1 and NCK2.The human NCK1 and NCK2 genes map to chromosomes 3q21 and 2q12, respectively.Synonyms: NCK adaptor protein 2, SH2/SH3 adaptor protein NCK-beta

Poids moléculaire: approx. 47 kDa

ID gène: 8440

NCBI Accession: NP_001004720

UniProt: O43639

Pathways: EGFR Signaling Pathway, Regulation of Actin Filament Polymerization