Ubiquitin anticorps

N° du produit ABIN271973

Il existe 2+ publications pour ce produit. L’anticorps anti-Ubiquitin Polyclonal Lapin est utilisé pour la détection de Ubiquitin dans des échantillons de Humain, Rat et Souris. Il a été validé pour WB et IHC (p).

Aperçu rapide pour Ubiquitin anticorps (ABIN271973)

Antigène: Ubiquitin (Ub)

Reactivité: Humain, Rat, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Ubiquitin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-Ubiquitin anticorps

Specificité: This antibody detects endogenous levels of Ubiquitin protein.

Purification: Affinity Chromatography using epitope-specific immunogen.

Information d'application

Indications d'application: ELISA: 1/20000approx. 1/40000. Immunohistochemistry: 1/50-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Références pour anticorps anti-Ubiquitin (ABIN271973)

Chen, Zhang, Chen, Bao, Wang, Zhu: "Electrical field stimulation induces cardiac fibroblast proliferation through the calcineurin-NFAT pathway." dans: Canadian journal of physiology and pharmacology, Vol. 90, Issue 12, pp. 1611-22, (2012) (PubMed).

Wang, Zhao, Qi, Gu, Rong, Mu, Zou, Tao, You, Guo: "Gambogic acid-induced degradation of mutant p53 is mediated by proteasome and related to CHIP." dans: Journal of cellular biochemistry, Vol. 112, Issue 2, pp. 509-19, (2011) (PubMed).

Détails sur Ubiquitin

Antigène: Ubiquitin (Ub)

Autre désignation: Ubiquitin

Sujet: Ubiquitin is a conserved polypeptide unit that plays an important role in the ubiquitin-proteasome pathway. Ubiquitin can be covalently linked to many cellular proteins by the ubiquitination process, which targets proteins for degradation by the 26S proteasome. Three components are involved in the target protein-ubiquitin conjugation process. Ubiquitin is first activated by forming a thiolester complex with the activation component E1, the activated ubiquitin is subsequently transfered to the ubiquitin-carrier protein E2, then from E2 to ubiquitin ligase E3 for final delivery to the epsilon-NH2 of the target protein lysine residue. The ubiquitin-proteasome pathway has been implicated in a wide range of normal biological processes and in disease-related abnormalities. Several proteins such as IkappaB, p53, cdc25a and Bcl-2 have been shown to be targets for the ubiquitination-proteosome process as part of regulation of cell cycle progression, differentiation, cell stress response and apoptosis.Synonyms: Polyubiquitin-B, UBB

ID gène: 7314

UniProt: P0CG47

Pathways: Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway