RPH3A
Reactivité: Humain, Souris, Rat
WB, IHC, ELISA, IF
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
ELISA: 1: 1000approx. 1: 5000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200. IF: 1: 50approx. 1: 200. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
The carboxy-terminal of rabphilin-3A consists of two C2 domains, A and B,and the amino-terminal (residues 45-170) contains a cysteine-rich rich region with two zinc finger motifs. Rabphilin-3A belongs to a family of other carboxyterminal type (C-type) tandem C2 proteins, which includes synaptotagmins and Doc2. Rabphilin is expressed in neuroendocrine cells and co-localizes with Rab3A on synaptic vesicles and chromaffin granules. Rabphilin-3A binds Rab3a/GTP/Mg+ within amino-terminal residues 45 and 170. Rabphilin-3A binds calcium ions and phosphatidylinositol 4,5-bisphophate containing lipid vesicles within its C2 domains. Rabphilin-3A is a positive regulator of calcium dependent exocytosis, while Rab3a is a negative regulator of exocytosis. Although rabphilin-3A associates with Rab3a, they seem to influence exocytosis independently of each other. Rabphilin-3A effects are likely mediated through interactions with an unknown factor that recognizes the Rab3 binding domain.Synonyms: Exophilin 1, Exophilin-1, KIAA0985, Rabphilin 3A