Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))
Specificité
This antibody recognizes the ~82k Rabphilin protein phosphorylated at Ser234. The immunolabeling of the Rabphilin band was completely blocked by the phosphopeptide used as antigen while the dephosphopeptide had no effect on the immunolabeling.
Réactivité croisée (Details)
Species reactivity (expected):Mouse. Species reactivity (tested):Rat.
Purification
Sequential Chromatography on phospho- and dephosphopeptide affinity columns.
Immunogène
Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser234 of Rabphilin 3A.
RPH3A
Reactivité: Humain, Souris, Rat
WB, IHC, ELISA, IF
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
Western blot: 1/1,000. Immunohistochemistry: 1/400. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Format
Liquid
Buffer
10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.
Conseil sur la manipulation
Avoid repeated freezing and thawing.
Stock
-20 °C
Stockage commentaire
Store the antibody undiluted (in aliquots) at-20 °C.
Rabphilin-3A is a peripheral membrane protein that binds Ca2+ and phospholipids and is attached to synaptic vesicle membranes (Geppert et al., 1994, Li et al., 1994). The expression of rabphilin 3A is reduced in an animal model of Huntington's disease(Smith et al., 2005). Rabphilin can be phosphorylated at Ser234 by both CAM kinase II and PKA and this phosphorylation has been suggested to regulate neuronal activity during development in a synapse-specific manner (Fykse et al., 1995,Foletti and Scheller, 2001).Synonyms: Exophilin 1, Exophilin-1, KIAA0985, Rabphilin 3A