Sp3 anticorps

N° du produit ABIN272209

Cet anticorps Lapin Polyclonal détecte spécifiquement Sp3 dans WB, IHC (p) et IF. Il présente une réactivité envers Humain, Souris et Rat.

Aperçu rapide pour Sp3 anticorps (ABIN272209)

Antigène: Sp3 (Sp3 Transcription Factor (Sp3))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Sp3 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Détail du produit anti-Sp3 anticorps

Specificité: This antibody detects endogenous levels of Sp3/4 protein. (region surrounding Gly659)

Réactivité croisée (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Pureté: > 95 % pure by SDS-PAGE

Information d'application

Indications d'application: ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: DO NOT FREEZE!

Stock: 4 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C.

Détails sur Sp3

Antigène: Sp3 (Sp3 Transcription Factor (Sp3))

Autre désignation: SP3 / SPR-2

Sujet: The Sp transcription factor family includes Sp1, Sp2, Sp3 (SPR-2) and Sp4(SPR-1). Sp transcription factors share similar structures but do not share similar functions. All four proteins contain a highly conserved DNA-binding domain composed of three zinc fingers at the C-terminus. Sp family members bind the consensus sequence GGGGCGGGGC and other closely related sequences which are known as GC boxes. Sp1, Sp3 and Sp4 share a high affinity for GC boxes while Sp2 does not. Sp2 only weakly binds to GT boxes. Sp1, Sp2 and Sp3 are ubiquitously expressed, while Sp4 is abundantly expressed in brain with limited expression in other tissues. Sp1 and Sp3,but not Sp2 or Sp4, interact with E2, a regulatory element for the β4 subunit of neuronal nicotinic acetylcholine receptors. Sp3 is the only Sp member to inhibit Sp1 and Sp4 mediated transcription. The gene encoding human Sp2 maps to chromosome 17p32.3.Synonyms: Transcription factor Sp3, Transcription factor Sp4

Poids moléculaire: approx. 85 kDa

ID gène: 6670

NCBI Accession: NP_001017371

UniProt: Q02447