NCBP1 anticorps

N° du produit ABIN272218

Cet anticorps Lapin Polyclonal détecte spécifiquement NCBP1 dans WB et EIA. Il présente une réactivité envers Humain, Souris et Rat.

Aperçu rapide pour NCBP1 anticorps (ABIN272218)

Antigène: NCBP1 (Nuclear Cap Binding Protein Subunit 1, 80kDa (NCBP1))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NCBP1 est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Détail du produit anti-NCBP1 anticorps

Specificité: This antibody detects endogenous levels of CBP80 protein. (region surrounding Arg5)

Réactivité croisée (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Pureté: > 95 % pure by SDS-PAGE

Information d'application

Indications d'application: ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: DO NOT FREEZE!

Stock: 4 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C.

Détails sur NCBP1

Antigène: NCBP1 (Nuclear Cap Binding Protein Subunit 1, 80kDa (NCBP1))

Autre désignation: NCBP1

Sujet: In eukaryotes, the majority of mRNAs have an m(7)G cap, which is added cotranscriptionally and plays a critical role in many aspects of mRNA metabolism. The effect of the cap on translation is mediated by the initiation factor eIF-4F, whereas the effect on pre-mRNA splicing involves a nuclear complex(CBC). CBC consists of two cap binding proteins CBP20 and CBP80, which mediate the stimulatory functions of the cap in pre-mRNA splicing, 3' end formation and U snRNA export. The genes CBC1 and CBC2 encode CBP80 and CBP20, respectively. CBP80 comprises three domains, each containing a MIF4G domain. CBP20 has an RNAP fold and associates with the second and third domains of CBP80. CBP also plays a role in nonsense-mediated decay(NMD), which eliminates mRNAs, which prematurely terminate translation.CBP80-bound mRNA undergoes a Synonyms: CBP80, NCBP, Nuclear cap-binding protein subunit 1

Poids moléculaire: approx. 88 kDa

ID gène: 4686

NCBI Accession: NP_002477

UniProt: Q09161

Pathways: Ribonucleoprotein Complex Subunit Organization, Photoperiodism, Methionine Biosynthetic Process