NCBP1
Reactivité: Souris
ELISA, IHC (p), IF (cc), IF (p), ICC, IHC (fro)
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1,0 mg/mL
Buffer
Phosphate buffered saline (PBS), pH 7.2., 15 mM sodium azide
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
DO NOT FREEZE!
Stock
4 °C
Stockage commentaire
Store the antibody undiluted at 2-8 °C.
Antigène
NCBP1
(Nuclear Cap Binding Protein Subunit 1, 80kDa (NCBP1))
anticorps CBP80, anticorps CG7035, anticorps Dmel\\CG7035, anticorps EG:84H4.3, anticorps ncbp, anticorps sto1, anticorps cbp80, anticorps AU014645, anticorps AW538051, anticorps NCBP, anticorps Sto1, anticorps ncbp1, anticorps ABA HYPERSENSITIVE 1, anticorps ATCBP80, anticorps CAP-BINDING PROTEIN 80, anticorps ENS, anticorps ENSALADA, anticorps NUCLEAR CAP-BINDING PROTEIN CBP80, anticorps T10F5.8, anticorps T10F5_8, anticorps cap binding protein 80, anticorps nuclear cap binding protein subunit 1, anticorps nuclear cap binding protein subunit 1 S homeolog, anticorps nuclear cap binding protein subunit 1, 80kDa, anticorps nuclear cap binding protein subunit 1 L homeolog, anticorps ARM repeat superfamily protein, anticorps Nuclear cap-binding protein subunit 1-A, anticorps Nuclear cap-binding protein subunit 1, anticorps Cbp80, anticorps ncbp1, anticorps ncbp1.S, anticorps NCBP1, anticorps Ncbp1, anticorps ncbp1.L, anticorps ABH1, anticorps nbp1a, anticorps ncbp-1
Sujet
In eukaryotes, the majority of mRNAs have an m(7)G cap, which is added cotranscriptionally and plays a critical role in many aspects of mRNA metabolism. The effect of the cap on translation is mediated by the initiation factor eIF-4F, whereas the effect on pre-mRNA splicing involves a nuclear complex(CBC). CBC consists of two cap binding proteins CBP20 and CBP80, which mediate the stimulatory functions of the cap in pre-mRNA splicing, 3' end formation and U snRNA export. The genes CBC1 and CBC2 encode CBP80 and CBP20, respectively. CBP80 comprises three domains, each containing a MIF4G domain. CBP20 has an RNAP fold and associates with the second and third domains of CBP80. CBP also plays a role in nonsense-mediated decay(NMD), which eliminates mRNAs, which prematurely terminate translation.CBP80-bound mRNA undergoes a Synonyms: CBP80, NCBP, Nuclear cap-binding protein subunit 1