NFIA anticorps

N° du produit ABIN272219

L’anticorps Lapin Polyclonal anti-NFIA a été validé pour WB et EIA. Il convient pour détecter NFIA dans des échantillons de Humain, Souris et Rat.

Aperçu rapide pour NFIA anticorps (ABIN272219)

Antigène: NFIA (Nuclear Factor I/A (NFIA))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NFIA est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Détail du produit anti-NFIA anticorps

Specificité: This antibody detects endogenous levels of NF-1 protein. (region surrounding Gln34)

Réactivité croisée (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Pureté: > 95 % pure by SDS-PAGE

Information d'application

Indications d'application: ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: DO NOT FREEZE!

Stock: 4 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C.

Détails sur NFIA

Antigène: NFIA (Nuclear Factor I/A (NFIA))

Autre désignation: NFIA

Sujet: NF-1, also designated CTF, consists of a family of CCAAT-box-binding proteins that stimulate DNA replication and activate transcription. Analysis of human NF-1 messenger RNA has revealed two forms of the NF-1 protein arising from an alternate splicing of a single NF-1 gene. NF-1 binds its consensus DNA element as a homodimer via an amino-terminal DNA-binding domain, and activates transcription through a putatively novel, proline-rich, carboxy-terminal transactivation domain. The NF-1 protein has been shown to recognize and bind the adenovirus type 2 promoter and activate transcription of herpes simplex virus thymidine kinase genes. The NF-1 consensus element has been found in the upstream promoter region of myriad eukaryotic genes, including that of Ha-Ras, α-globin, HSP 70, GRP 78, Histone H1,myelin basic protein and in the Xenopus laevis vitellogenin gene promoter.Synonyms: CCAAT-box-binding transcription factor, CTG, NF-I/A, NFI-A, Nuclear factor 1 A-type, TGGCA-binding protein

Poids moléculaire: approx. 55 kDa

ID gène: 4774

NCBI Accession: NP_001128145

UniProt: Q12857