PFKFB1 anticorps

N° du produit ABIN272241

Cet anticorps anti-PFKFB1 Polyclonal Lapin (ABIN272241) détecte spécifiquement PFKFB1 dans WB. L’anticorps est réactif avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour PFKFB1 anticorps (ABIN272241)

Antigène: PFKFB1 (6-phosphofructo-2-Kinase/fructose-2,6-Biphosphatase 1 (PFKFB1))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PFKFB1 est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-PFKFB1 anticorps

Specificité: This antibody detects endogenous levels of PFKFB1/4 protein. (region surrounding Glu349)

Réactivité croisée (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Pureté: > 95 % pure by SDS-PAGE

Information d'application

Indications d'application: ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: DO NOT FREEZE!

Stock: 4 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C.

Détails sur PFKFB1

Antigène: PFKFB1 (6-phosphofructo-2-Kinase/fructose-2,6-Biphosphatase 1 (PFKFB1))

Autre désignation: PFKFB1

Sujet: PFKFB (6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase) is a bifunctional enzyme, having both kinase and phosphatase activities residing on the same enzyme subunit but having distinct active sites. PFKFB regulates the steady-state concentration of fructose-2,6-bisphosphate, a potent activator of a key regulatory enzyme of glycolysis, phosphofructokinase. To date, four PFKFB isozymes (PFKFB 1-4) have been described, which show differences in their tissue distribution and kinetic properties in response to allosteric effectors and hormonal signals. Among the PFKFB's PFKFB3 has the highest kinase:phosphatase ratio, in part because it lacks the characteristic serine phosphorylation site near the N-terminal that down-modulates kinase activity. PFKFB3 was first described in the rapidly growing placenta. The glucolitic rate in placenta is accelerated by anoxia and by maternal diabetes. Cancer cells maintain a high glycolytic rate even in the presence of oxygen, a phenomenon known as the Warburg effect. The glycolytic rate in the placenta, another fast-growing tissue, is accelerated by anoxia and by maternal diabetes.Synonyms: 6-biphosphatase 1, 6-phosphofructo-2-kinase/fructose-2, F6PK, PFK-2 liver, PFK/FBPase 1, PFRX

Poids moléculaire: approx. 54 kDa

ID gène: 5207

NCBI Accession: NP_002616

UniProt: P16118

Pathways: Regulation of Carbohydrate Metabolic Process