14-3-3 theta anticorps

N° du produit ABIN272267

L’anticorps anti-14-3-3 theta Polyclonal Lapin est utilisé pour la détection de 14-3-3 theta dans des échantillons de Humain, Souris et Rat. Il a été validé pour WB, IF et IHC (p).

Aperçu rapide pour 14-3-3 theta anticorps (ABIN272267)

Antigène: 14-3-3 theta (YWHAQ)

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp 14-3-3 theta est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-14-3-3 theta anticorps

Specificité: This antibody detects endogenous levels of 14-3-3 θ protein. (region surrounding Gln67)

Réactivité croisée (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Pureté: > 95 % pure by SDS-PAGE

Information d'application

Indications d'application: ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1,0 mg/mL

Buffer: Phosphate Buffered Saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: DO NOT FREEZE!

Stock: 4 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C.

Détails sur 14-3-3 theta

Antigène: 14-3-3 theta (YWHAQ)

Autre désignation: 14-3-3 Protein theta

Sujet: 14-3-3 proteins regulate many cellular processes relevant to cancer biology,notably apoptosis, mitogenic signaling and cell-cycle checkpoints. Seven isoforms comprise this family of signaling intermediates, denoted 14-3-3 β, γ, ε, ζ, η, θ and σ. 14-3-3 proteins form dimers that present two binding sites for ligand proteins, thereby bringing together two proteins that may not otherwise associate. These ligands largely share a 14-3-3 consensus binding motif and exhibit serine/threonine phosphorylation. 14-3-3 proteins function in broad regulation of these ligand proteins, by cytoplasmic sequestration, occupation of interaction domains and import/export sequences, prevention of degradation, activation/repression of enzymatic activity and facilitation of protein modification, and thus loss of expression contributes to a vast array of pathogenic cellular activitiesSynonyms: 14-3-3 protein T-cell, 14-3-3 protein tau, Protein HS1, YWHAQ

Poids moléculaire: approx. 28 kDa

ID gène: 10971

NCBI Accession: NP_006817

UniProt: P27348

Pathways: Apoptose, Myometrial Relaxation and Contraction