CDC20 anticorps

N° du produit ABIN272282

L’anticorps anti-CDC20 Polyclonal Lapin est utilisé pour la détection de CDC20 dans des échantillons de Humain, Souris et Rat. Il a été validé pour WB et IHC (p).

Aperçu rapide pour CDC20 anticorps (ABIN272282)

Antigène: CDC20 (Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CDC20 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-CDC20 anticorps

Specificité: This antibody detects endogenous levels of Cdc20 protein. (region surrounding Gln105)

Réactivité croisée (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Pureté: > 95 % pure by SDS-PAGE

Information d'application

Indications d'application: ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: DO NOT FREEZE!

Stock: 4 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C.

Détails sur CDC20

Antigène: CDC20 (Cell Division Cycle 20 Homolog (S. Cerevisiae) (CDC20))

Autre désignation: CDC20

Sujet: Cell cycle progression is controlled at a point late in G1 designated Start. Passage through Start requires the activity of the cyclin-dependent protein kinase Cdc28. Transition from G1 to S phase requires the association of Cdc28 with members of the G1 cyclin family. Exit from mitosis and initiation of the next cell cycle requires a complex of proteins designated the anaphase-promoting complex (APC). This complex consists of two proteins,Cdc16 and Cdc27 (also referred to as Snb1), which are involved in limiting DNA replication to once per cell cycle. Cdc23, another component of the APC, is required for both entering and exiting anaphase, and is important for the proper separation of sister chromatids. The APC is thought to be stabilized by Cdc26 (also known as Scd26). In addition to these APC proteins, Cdc5 is also required for completion of mitosis. In contrast, Cdc20 acts as a DNA-damage induced checkpoint, preventing mitosis when DNA damage has occurred.Synonyms: Cell division cycle protein 20 homolog, p55CDC

Poids moléculaire: approx. 55 kDa

ID gène: 991

NCBI Accession: NP_001246

UniProt: Q12834