ACTN3 anticorps

N° du produit ABIN272284

Cet anticorps Lapin Polyclonal détecte spécifiquement ACTN3 dans WB et IF. Il présente une réactivité avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour ACTN3 anticorps (ABIN272284)

Antigène: ACTN3 (Actinin, alpha 3 (ACTN3))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ACTN3 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Détail du produit anti-ACTN3 anticorps

Specificité: This antibody detects endogenous levels of Actinin-α3 protein. (region surrounding Glu25)

Réactivité croisée (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Pureté: > 95 % pure by SDS-PAGE

Information d'application

Indications d'application: ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: DO NOT FREEZE!

Stock: 4 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C.

Détails sur ACTN3

Antigène: ACTN3 (Actinin, alpha 3 (ACTN3))

Autre désignation: alpha-Actinin-3 / ACTN3

Sujet: The spectrin gene family encodes a diverse group of cytoskeletal proteins that include spectrins, dystrophins and α-actinins. There are four tissue-specific α-actinins, namely α-actinin-1, α-actinin-2, α-actinin-3 and α-actinin-4, which are localized to muscle and non-muscle cells, including skeletal, cardiac and smooth muscle cells, as well as within the cytoskeleton. Each α-actinin protein contains one Actin-binding domain, two calponin-homology domains, two EF-hand domains and four spectrin repeats, through which they function as bundling proteins that can cross-link F-Actin, thus anchoring Actin to a variety of intracellular structures. Defects in the gene encoding α-actinin-4 are the cause of focal segmental glomerulosclerosis 1 (FSGS1), a common renal lesion characterized by decreasing kidney function and, ultimately, renal failure.Synonyms: Alpha actinin skeletal muscle isoform 3, F-actin cross-linking protein

Poids moléculaire: approx. 103 kDa

ID gène: 89

NCBI Accession: NP_001095

UniProt: Q08043

Pathways: Cell-Cell Junction Organization