DFFA anticorps

N° du produit ABIN272297

L’anticorps Lapin Polyclonal anti-DFFA a été validé pour WB, IHC (p) et EIA. Il convient pour détecter DFFA dans des échantillons de Humain, Souris et Rat.

Aperçu rapide pour DFFA anticorps (ABIN272297)

Antigène: DFFA (DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp DFFA est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Détail du produit anti-DFFA anticorps

Specificité: This antibody detects endogenous levels of ICAD protein. (region surrounding Gln177)

Réactivité croisée (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Pureté: > 95 % pure by SDS-PAGE

Information d'application

Indications d'application: ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: DO NOT FREEZE!

Stock: 4 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C.

Détails sur DFFA

Antigène: DFFA (DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA))

Autre désignation: DFFA / ICAD

Sujet: The CED/ICE family of cysteine proteases plays a pivotal role in mediating apoptosis through the proteolysis of specific targets. Among the targets are poly (ADP-ribose) polymerase (PARP), gelsolin, DFF-45/ICAD and the nuclear lamins. PARP is a 112 kDa nuclear protein that is specifically cleaved by CPP32 and Mch2, but not by ICE, into a signature 85 kDa apoptotic fragment. Gelsolin is cleaved by CPP32 to an active form that severs actin filaments in a Ca++-independent manner. In addition to binding actin, gelsolin can form complexes with fibronectin, which may be important for localizing gelsolin to inflammatory sites. DFF-45/ICAD, the 45 kDa subunit of DNA fragmentation factor, is cleaved by CPP32 to generate an active factor that induces DNA fragmentation. The 70 kDa nuclear Lamin A is cleaved by Mch2, but not CPP32. Nuclear Lamin B is fragmented as a consequence of apoptosis by an unidentified member of the ICE family.Synonyms: DFF-45, DFF1, DFF45, DNA fragmentation factor 45 kDa subunit, DNA fragmentation factor subunit alpha, Inhibitor of CAD

Poids moléculaire: approx. 36 kDa

ID gène: 13347

NCBI Accession: NP_034174

UniProt: O54786

Pathways: Apoptose, Caspase Cascade in Apoptosis