CD34 anticorps (N-Term)

N° du produit ABIN2739286

Cet anticorps anti-CD34 est un anticorps Souris Monoclonal détectant CD34 dans FACS, WB, IHC, IF, IHC (p) et IP. Adapté pour Humain, Cynomolgus et Macaque rhésus.

Aperçu rapide pour CD34 anticorps (N-Term) (ABIN2739286)

Antigène: CD34

Reactivité: Humain, Cynomolgus, Macaque rhésus

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD34 est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Clone: QBEnd-10

Détail du produit anti-CD34 anticorps

Épitope: N-Term

Specificité: Human CD34

Aucune reactivité croisée: Boeuf (Vache), Chien, Rat (Rattus), Mouton

Purification: Protein G purified

Immunogène: A detergent solubilized vesicular suspension prepared from human term placenta.

Isotype: IgG1 kappa

Information d'application

Indications d'application: Approved: Flo (0.5 - 1 μg/10 x10⁶), IF (0.5 - 1 μg/mL), IHC, IHC-P (0.5 - 1 μg/mL), IP, WB (0.25 - 0.5 μg/mL)

Usage: This antibody recognizes a single chain, transmembrane, heavily glycosylated protein of 90-120  kDa, which is identified as CD34. On the basis of differential sensitivity to degradation by specific enzymes, epitopes of monoclonal antibodies to CD34 are classified into three main categories, class I, class II and class III. This is a class II antibody whose epitope is resistant to neuraminidase but sensitive to glycoprotease and chymopapain. CD34 expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. Its expression is also found in vascular endothelium. Additionally, it appears that proliferating endothelial cells express this molecule more than the non-proliferating endothelial cells. CD34 antibody labels > 85 % of angiosarcoma and Kaposi's sarcoma, but with a lower specificity.

Commentaires:

Target Species of Antibody: Human

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 mg/mL BSA, 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store at 2°C to 8°C.

Détails sur CD34

Antigène: CD34

Autre désignation: CD34

Sujet: Name/Gene ID: CD34

Synonyms: CD34, CD34 Molecule, CD34 antigen

ID gène: 947