CD8 alpha anticorps (Cy7,PE)

N° du produit ABIN2739737

Cet anticorps Rat Monoclonal détecte spécifiquement CD8 alpha dans FACS et ELISA. Il présente une réactivité avec des échantillons de Souris.

Aperçu rapide pour CD8 alpha anticorps (Cy7,PE) (ABIN2739737)

Antigène: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp CD8 alpha est conjugé à/à la Cy7,PE

Application: Flow Cytometry (FACS), ELISA

Clone: 53-6-72

Détail du produit anti-CD8 alpha anticorps (Cy7,PE)

Specificité: Mouse CD8.

Purification: Protein G purified

Immunogène: Mouse thymocytes/splenocytes.

Type of Immunogen: Cells

Isotype: IgG2a kappa

Information d'application

Indications d'application: Approved: ELISA, Flo

Usage: Reagent was tested for binding to ACKlysed murine splenocytes in FACS. Five x 105 splenocytes per tube were washed and pre incubated with 20 μL of 300 μg/mL Mouse IgG (to block non specific binding), after which they were incubated 45 minutes on ice with 80 μL of anti-Mouse CD8/PE-Cy7 at a 1:50 dilution (5 μg/mL). They were then washed three times, fixed and analyzed by FACS. An 8.3 % sub population of the cells stained positive with a mean shift of 1.4 log10 fluorescent units when compared to background. Binding was blocked when cells were pre incubated 10 minutes with 20 μL of 0.5 mg/mL anti-mouse CD8 antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Commentaires:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: 50 mM sodium phosphate,  pH 7.5, 500 mM potassium chloride, 150 mM sodium chloride, 0.2 % BSA, 0.04 % sodium azide, 15 % glycerol

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.

Stock: 4 °C

Stockage commentaire: Store at 4°C. Do not freeze. Protect from light.

Détails sur CD8 alpha

Antigène: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Autre désignation: CD8A / CD8 alpha

Sujet: Name/Gene ID: CD8A

Synonyms: CD8A, CD8a molecule, CD8a antigen, OKT8 T-cell antigen, Leu2 T-lymphocyte antigen, T8 T-cell antigen, T cell co-receptor, p32, CD8, Leu2, T-cell antigen Leu2

ID gène: 925

Pathways: TCR Signaling