alpha Tubulin anticorps

N° du produit ABIN2745507

L’anticorps Souris Monoclonal anti-alpha Tubulin a été validé pour WB et ICC. Il convient pour détecter alpha Tubulin dans des échantillons de Toutes les espèces.

Aperçu rapide pour alpha Tubulin anticorps (ABIN2745507)

Antigène: alpha Tubulin (TUBA1)

Reactivité: Toutes les espèces

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp alpha Tubulin est non-conjugé

Application: Western Blotting (WB), Immunocytochemistry (ICC)

Clone: TEU318

Détail du produit anti-alpha Tubulin anticorps

Specificité: Detects K40 acetylation of alpha-tubulin, signal specifically increased by modification with tubulin acetyl transferase alpha-TAT1.

Réactivité croisée: Toutes les espèces

Pureté: >95 % (SDS-PAGE)

Immunogène: Tubulin of the Ciliate Euplotes eluted from the 55kDa band of SDS gel.

Isotype: IgG1

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: In PBS containing 10 % glycerol and 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 year after receipt when stored at -20°C.

Date de péremption: 12 months

Détails sur alpha Tubulin

Antigène: alpha Tubulin (TUBA1)

Autre désignation: alpha-Tubulin

Sujet: Microtubules are key elements of the eukaryotic cytoskeleton that dynamically assemble from heterodimers of alpha- and beta-tubulin. Two different mechanisms can generate microtubule diversity: the expression of different alpha- and beta-tubulin genes, referred to as tubulin isotypes, and the generation of posttranslational modifications (PTMs) on alpha- and beta-tubulin. Tubulin PTMs include the well-known acetylation or phosphorylation, and others that have so far mostly been found on tubulin, detyrosination/tyrosination, polyglutamylation and polyglycylation. These PTMs might have evolved to specifically regulate tubulin and microtubule functions. Tubulin acetylation was discovered on K40 of flagellar alpha-tubulin in Chlamydomonas reinhardtii and is generally enriched on stable microtubules in cells. It is located on the microtubule lumenal surface. As a result of its localization at the inner face of microtubules, K40 acetylation might rather affect the binding of microtubule inner proteins, a poorly characterized family of proteins. Functional experiments in cells have further suggested that K40 acetylation regulates intracellular transport by regulating the traffic of kinesin motors probably by indirect mechanisms. Acetyltransferase alpha-Tat1 (or Mec-17) specifically acetylate alpha-tubulin K40. Acetylation of tubulin by alpha-Tat1 accumulates selectively in stable, long-lived microtubules thus explaining the link between this posttranslational modication and stable microtubules in cells. However, the direct cellular function of K40 acetylation on microtubules is still unclear.

Pathways: Dynamique des Microtubules