C6orf150 anticorps (AA 450-500)

N° du produit ABIN2746464

Détail du produit anti-C6orf150 anticorps

Antigène: C6orf150 (Chromosome 6 Open Reading Frame 150 (C6orf150))

Épitope: AA 450-500

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp C6orf150 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

Réactivité croisée: Souris, Rat (Rattus)

Attributs du produit: Cyclic-GAS-selective antibodies were generated against peptides taken from the mouse/rat and human cGAS protein. The cGAS-selective antibodies were affinity purified on an immobilized antigen based affinity matrices, the isolated antibodies were then stabilized in antibody stabilization buffer for long-term storage. The anti-cGAS-selective antibodies are fully characterized for applications in western blotting and ELISA at the recommended dilutions. The supplier provides cGAS western blot positive controls samples in SDS-PAGE sample buffer.
Synonyms: cGMP Synthase, h-cGAS, Mab-21 domain-containing protein 1, chromosome 6 open reading frame 150, C7Orf150, MB21D1

Purification: Affinity Purified

Immunogène: Synthetic peptide taken within amino acid region 450-500 on mouse cGAS protein.

Isotype: IgG

Information d'application

Indications d'application: Antibodies were tested in ELISA and western blotting applications at 1:500 dilution using ABIN1686780 samples. Antibody dilutions for these antibodies are for reference only, investigators are expected to determine the optimal conditions. Application of this antibody in other protocols has not yet tested.
WB: > 1:500
IMM & IP pull-down assays: N.D.
IHC: N.D. .
Investigators using this antibody in protocols other than listed above can request a complimentary sample of this antibody. N.D. not necessarily means the antibody is not suitable for that application, it simply means we have not yet characterized the antibody in that application.
The antibody hcGAS-112AP labels a strong band of cGAS of 64 kDa in ABIN1686780 samples and in several cell lines. The antibody also labels weakly 78 and 90 kDa bands, the identity of these bands are not known at this time.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.6 μg/μL

Stock: -20 °C

Stockage commentaire: Storage of very dilute antibody solutions is not recommended.

Références pour anticorps anti-C6orf150 (ABIN2746464)

Doi, Iijima, Kano, Ishizaka: "Viral protein R of HIV type-1 induces retrotransposition and upregulates glutamate synthesis by the signal transducer and activator of transcription 1 signaling pathway." dans: Microbiology and immunology, Vol. 59, Issue 7, pp. 398-409, (2016) (PubMed).

Détails sur C6orf150

Antigène: C6orf150 (Chromosome 6 Open Reading Frame 150 (C6orf150))

Autre désignation: C6orf150 (C6orf150 Produits)

Synonymes: anticorps C6orf150, anticorps cGAS, anticorps h-cGAS, anticorps Mab-21 domain containing 1, anticorps cyclic GMP-AMP synthase, anticorps MB21D1, anticorps CGAS

Sujet: The cyclic guanosine monophosphate-adenosime monophosphate (GMP-AMP) synthase (cGAS) belongs to the nuceotidyltransferase family, catalyzes the formation of cGMP-AMP (cGAMP) from ATP and GTP. The prsence of cytosolic DNA in the cytoplasm of mammalian cells is a danger signal that triggers the most immune responses which produces the type I interferons. The DNA signaling pathway requires an adapter protein STING and transcription factor IRF3. The mechanism thru which interferons are induced by cytosolic DNA involves cyclic GMP-AMP synthase which binds and activates the endoplasmic reticulum adapter protein STING, also known as MITA, MPYS, or ERIS that activates the transcription activity of interferon syntheses. The STING recruits and activates the cytosolic kinase IKK and TBK1, which activates the transcription factor NF-kB and transcription fctor IRF3. The NF-kB and IRF3 enters the nucleus and in concert induce interferons and other cytokines. The DNA-dependent RNA Polymerease III also acts as a sensor and synthesize AT-rich DNA such as poly(dA:dT) in to n RNA ligand that stimulates RIG-1 pathway to induce interferons. Since most DNA do not activate DNA-dependent RNA polymerase and downstream RIG1 pathways, but instead activate STING pathway via cGAS and cGAMP stimulation for interferon induction. DNA can be introduced by viral or bacterial infection, or leakage form nucleus or mitochondria under some pathological conditions that cause auto immune disease like Lupus. Cytosolic cGMP-AMP synthesized in response to DNA by cGAS acts as a second messenger in innate immune signaling. cGAS gene encodes for a 522 AA protein that has a Mab-21 motif commonly found in C. elegans that is required for choice of alternate cell fates in male tail cells .

UniProt: Q8C6L5

Pathways: Activation of Innate immune Response