MCAM anticorps

N° du produit ABIN2749195

Cité dans 3 publications. Cet anticorps anti-MCAM Monoclonal Souris (Clone P1H12) (ABIN2749195) détecte spécifiquement MCAM dans FACS, WB, IP, IHC (p), ICC et IHC (fro). L’anticorps est réactif avec des échantillons de Humain, Souris, Lapin et Chien.

Aperçu rapide pour MCAM anticorps (ABIN2749195)

Antigène: MCAM (Melanoma Cell Adhesion Molecule (MCAM))

Reactivité: Humain, Souris, Lapin, Chien

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MCAM est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: P1H12

Détail du produit anti-MCAM anticorps

Fonction: Anti-Hu CD146 Purified

Aucune reactivité croisée: Rat

Réactivité croisée (Details): Human, Mouse, Canine (Dog), Rabbit

Attributs du produit: The mouse monoclonal antibody P1H12 recognizes an extracellular epitope of CD146, a 118 kDa transmembrane glycoprotein expressed on epithelial and endothelial cells, fibroblasts, multipotent mesenchymal stromal cells, melanoma cells, activated T cells and activated keratinocytes.

Purification: Purified by protein-A affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

Immunogène: cultured human umbilical cells

Isotype: IgG1

Information d'application

Indications d'application: Western blotting: Nonreducing conditions.
Flow cytometry: Recommended dilution: 1-4 μg/mL

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Références pour anticorps anti-MCAM (ABIN2749195)

Kamstock, Guth, Elmslie, Kurzman, Liggitt, Coro, Fairman, Dow: "Liposome-DNA complexes infused intravenously inhibit tumor angiogenesis and elicit antitumor activity in dogs with soft tissue sarcoma." dans: Cancer gene therapy, Vol. 13, Issue 3, pp. 306-17, (2006) (PubMed).

Solovey, Gui, Chang, Enenstein, Browne, Hebbel: "Identification and functional assessment of endothelial P1H12." dans: The Journal of laboratory and clinical medicine, Vol. 138, Issue 5, pp. 322-31, (2001) (PubMed).

Solovey, Lin, Browne, Choong, Wayner, Hebbel: "Circulating activated endothelial cells in sickle cell anemia." dans: The New England journal of medicine, Vol. 337, Issue 22, pp. 1584-90, (1997) (PubMed).

Détails sur MCAM

Antigène: MCAM (Melanoma Cell Adhesion Molecule (MCAM))

Autre désignation: CD146

Sujet: Melanoma cell adhesion molecule,CD146, also known as MCAM (melanoma cell adhesion molecule) or MUC18, is a heavily glycosylated transmembrane glycoprotein with more than 50 % of the mass from carbohydrates. It is expressed on epithelial and endothelial cells, fibroblasts, multipotent mesenchymal stromal cells, activated T cells and activated keratinocytes, and on some cancer cells, especially melanoma. The presence of CD146 on circulating blood cells has been confined to the activated T cells rather than circulating endothelial cells. CD146 mediates heterophilic cell adhesion and regulates monocyte transendothelial migration.,MelCAM, MCAM, MUC18

ID gène: 4162

UniProt: P43121