Topoisomerase I anticorps

N° du produit ABIN2763824

L’anticorps anti-Topoisomerase I Polyclonal Lapin est utilisé pour la détection de Topoisomerase I dans des échantillons de Humain. Il a été validé pour IHC (p).

Aperçu rapide pour Topoisomerase I anticorps (ABIN2763824)

Antigène: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Topoisomerase I est non-conjugé

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-Topoisomerase I anticorps

Réactivité croisée: Humain

Attributs du produit: DNA Topoisomerase 1 releases the supercoiling and torsional tension of DNA introduced during DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. It introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells.

Purification: Antigen Immunoaffinity Purification

Immunogène: Synthetic peptide made to human DNA Topoisomerase 1

Isotype: Ig Fraction

Information d'application

Indications d'application: Antibody can be used for immunohistochemistry at 5-10 μg/mL on paraffin-embedded tissues. Optimal concentration should be evaluated by serial dilutions.

Restrictions: For Research Use only

Stockage

Buffer: Provided as solution in phosphate buffered saline with 0.08 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Détails sur Topoisomerase I

Antigène: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Autre désignation: DNA Topoisomerase 1

UniProt: P11387

Pathways: Caspase Cascade in Apoptosis, Stem Cell Maintenance