RAD18 anticorps (Middle Region)

N° du produit ABIN2774765

Cet anticorps anti-RAD18 est un anticorps Lapin Polyclonal détectant RAD18 dans WB. Adapté pour Humain, Rat, Souris, Porc, Lapin, Boeuf (Vache), Chien, Cheval et Cobaye. Ce Primary Antibody a été cité dans 1 publication.

Aperçu rapide pour RAD18 anticorps (Middle Region) (ABIN2774765)

Antigène: RAD18 (E3 ubiquitin-protein ligase RAD18 (RAD18))

Reactivité: Humain, Rat, Souris, Porc, Lapin, Boeuf (Vache), Chien, Cheval, Cobaye

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp RAD18 est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-RAD18 anticorps

Épitope: Middle Region

Séquence: KQVTKVDCPV CGVNIPESHI NKHLDSCLSR EEKKESLRSS VHKRKPLPKT

Homologie: Cow: 100%, Dog: 86%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%

Attributs du produit: This is a rabbit polyclonal antibody against RAD18. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogène: The immunogen is a synthetic peptide directed towards the middle region of human RAD18

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator.

Commentaires:

Antigen size: 495 AA

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Références pour anticorps anti-RAD18 (ABIN2774765)

Tsuji, Watanabe, Araki, Shinohara, Yamagata, Tsurimoto, Hanaoka, Yamamura, Yamaizumi, Tateishi: "Recognition of forked and single-stranded DNA structures by human RAD18 complexed with RAD6B protein triggers its recruitment to stalled replication forks." dans: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 13, Issue 4, pp. 343-54, (2008) (PubMed).

Détails sur RAD18

Antigène: RAD18 (E3 ubiquitin-protein ligase RAD18 (RAD18))

Autre désignation: RAD18

Sujet: RAD18 is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, RAD18 is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens. The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: RNF73
Protein Interaction Partner: TAX1BP1, UBC, SHPRH, RAD18, SIVA1, USP2, UBE2B, UBE2A, HLTF, PCNA, BPLF1, USP5, DBF4, BRCA1, DZIP3, POLH, DTL, Ankrd32, MLKL, ECT2, RPL10A, TRIM23, PCGF6, TRIM8, RFC2, CPD, SPRTN, ZMYM2, MAPK8, POLN, WRNIP1, gag-pol, ORC2, SUMO2, POLK, NBN, Smc6, HIST1H2A
Protein Size: 495

Poids moléculaire: 56 kDa

ID gène: 56852

NCBI Accession: NM_020165, NP_064550

UniProt: Q9NS91