MCM4 anticorps (Middle Region)

N° du produit ABIN2774884

Cet anticorps anti-MCM4 est un anticorps Polyclonal produit chez le Lapin, détectant MCM4 dans WB et IHC. Convient pour Humain, Souris, Rat, Lapin, Boeuf (Vache), Cobaye, Chien, Cheval et Poisson zèbre (Danio rerio).

Aperçu rapide pour MCM4 anticorps (Middle Region) (ABIN2774884)

Antigène: MCM4 (Minichromosome Maintenance Deficient 4 (MCM4))

Reactivité: Humain, Souris, Rat, Lapin, Boeuf (Vache), Cobaye, Chien, Cheval, Poisson zèbre (Danio rerio)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MCM4 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-MCM4 anticorps

Épitope: Middle Region

Séquence: VYKTHIDVIH YRKTDAKRLH GLDEEAEQKL FSEKRVELLK ELSRKPDIYE

Homologie: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 85%

Attributs du produit: This is a rabbit polyclonal antibody against MCM4. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogène: The immunogen is a synthetic peptide directed towards the middle region of human MCM4

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator.

Commentaires:

Antigen size: 863 AA

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Détails sur MCM4

Antigène: MCM4 (Minichromosome Maintenance Deficient 4 (MCM4))

Autre désignation: MCM4

Sujet: The protein encoded by MCM4 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. The MCM4 gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks.
Alias Symbols: CDC21, CDC54, hCdc21, P1-CDC21
Protein Interaction Partner: HUWE1, UBC, PCNA, SUMO2, FKBP5, MCMBP, MDM2, POLK, CLUH, NCKAP1, CAP1, CAP2, EIF3B, MCM2, KPNA3, PRMT1, FKBP4, MCM7, MCM6, FBXW4, FBXO46, ITGA4, CDC5L, gag-pol, ORC6, CCNA1, VCAM1, DBF4, CDC7, LIG4, MCM5, MCM3, DCTN2, SF3A1, UBD, NEDD8, TONSL, SIRT7, ASF1
Protein Size: 863

Poids moléculaire: 95 kDa

ID gène: 4173

NCBI Accession: NM_005914, NP_005905

UniProt: P33991

Pathways: Réparation de l'ADN, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA