GAPDH anticorps (N-Term)

N° du produit ABIN2778645

Cet anticorps Lapin Polyclonal détecte spécifiquement GAPDH dans WB et IHC. Il présente une réactivité envers Humain, Souris, Rat, Lapin, Chien, Mouton, Boeuf (Vache), Cheval, Chévre et Cobaye et a été mentionné dans 2+ publications.

Aperçu rapide pour GAPDH anticorps (N-Term) (ABIN2778645)

Antigène: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reactivité: Humain, Souris, Rat, Lapin, Chien, Mouton, Boeuf (Vache), Cheval, Chévre, Cobaye

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GAPDH est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-GAPDH anticorps

Épitope: N-Term

Séquence: IDLNYMVYMF QYDSTHGKFH GTVKAENGKL VINGNPITIF QERDPSKIKW

Homologie: Cow: 93%, Dog: 100%, Goat: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 93%, Rabbit: 100%, Rat: 100%, Sheep: 100%

Attributs du produit: This is a rabbit polyclonal antibody against GAPDH. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogène: The immunogen is a synthetic peptide directed towards the N terminal region of human GAPDH

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator.

Commentaires:

Antigen size: 335 AA

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Références pour anticorps anti-GAPDH (ABIN2778645)

Kalinec, Thein, Parsa, Yorgason, Luxford, Urrutia, Kalinec: "Acetaminophen and NAPQI are toxic to auditory cells via oxidative and endoplasmic reticulum stress-dependent pathways." dans: Hearing research, Vol. 313, pp. 26-37, (2014) (PubMed).

Fu, Mukhamedova, Ip, DSouza, Henley, DiTommaso, Kesani, Ditiatkovski, Jones, Lane, Jennings, Smyth, Kile, Sviridov: "ABCA12 regulates ABCA1-dependent cholesterol efflux from macrophages and the development of atherosclerosis." dans: Cell metabolism, Vol. 18, Issue 2, pp. 225-38, (2013) (PubMed).

Détails sur GAPDH

Antigène: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Autre désignation: GAPDH

Sujet: GAPDH catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome.Glyceraldehyde-3-phosphate dehydrogenase catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. A GAPD pseudogene has been mapped to Xp21-p11 and 15 GAPD-like loci have been identified. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: G3PD, GAPD, MGC88685
Protein Interaction Partner: FUS, HUWE1, UBC, SUMO2, SUMO3, STAU1, MDM2, GAPDH, ASB15, ASB16, RPA3, RPA2, RPA1, EED, SNCA, rev, HSPA9, HSPA1L, GAS7, TUBA1A, TUFM, RAP1GDS1, PPP5C, PPIB, YWHAG, TUBB, TUBA1C, PRPF40A, UBQLN1, DCPS, MAT2B, ACOT7, METAP2, FERMT2, GBP1, EEF1A1, TYMP, DFFA
Protein Size: 335

Poids moléculaire: 36 kDa

ID gène: 2597

NCBI Accession: NM_002046, NP_002037

UniProt: Q2TSD0