DFFA anticorps (N-Term)

N° du produit ABIN2779329

Cet anticorps anti-DFFA Polyclonal Lapin (ABIN2779329) détecte spécifiquement DFFA dans WB. L’anticorps est réactif avec des échantillons de Humain, Souris, Rat, Chien, Boeuf (Vache), Cobaye et Cheval.

Aperçu rapide pour DFFA anticorps (N-Term) (ABIN2779329)

Antigène: DFFA (DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA))

Reactivité: Humain, Souris, Rat, Chien, Boeuf (Vache), Cobaye, Cheval

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp DFFA est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-DFFA anticorps

Épitope: N-Term

Fonction: DFFA Antibody - N-terminal region

Séquence: MEVTGDAGVP ESGEIRTLKP CLLRRNYSRE QHGVAASCLE DLRSKACDIL

Homologie: Cow: 92%, Dog: 83%, Guinea Pig: 92%, Horse: 92%, Human: 100%, Mouse: 83%, Rat: 92%

Attributs du produit: This is a rabbit polyclonal antibody against DFFA. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogène: The immunogen is a synthetic peptide directed towards the N terminal region of human DFFA

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8099047

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: prevent freeze-thaw cycles

Stock: 4 °C,-20 °C

Stockage commentaire: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Détails sur DFFA

Antigène: DFFA (DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA))

Autre désignation: DFFA

Sujet:

Targeted Subunit: alpha

Background Information: Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis.Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Gene Name: DNA fragmentation factor, 45 kDa, alpha polypeptide

Alternative Symbols: DFF1, ICAD, DFF-45

Protein Name: DNA fragmentation factor subunit alpha

Poids moléculaire: 37kDa

ID gène: 1676

NCBI Accession: NP_004392

UniProt: O00273

Pathways: Apoptose, Caspase Cascade in Apoptosis