Deoxyuridine Triphosphatase (DUT) (N-Term) anticorps

N° du produit ABIN2782437

L’anticorps Lapin Polyclonal anti- a été validé pour WB et IHC. Il convient pour détecter dans des échantillons de Humain. Il y a 1 publication disponible.

Aperçu rapide pour Deoxyuridine Triphosphatase (DUT) (N-Term) anticorps (ABIN2782437)

Antigène: Deoxyuridine Triphosphatase (DUT)

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Inconjugué

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit

Épitope: N-Term

Séquence: AAVLSGPGPP LGRAAQHGIP RPLSSAGRLS QGCRGASTVG AAGWKGELPK

Homologie: Human: 100%

Attributs du produit: This is a rabbit polyclonal antibody against DUT. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogène: The immunogen is a synthetic peptide directed towards the N terminal region of human DUT

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator.

Commentaires:

Antigen size: 252 AA

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Référence

Tóth, Varga, Kovács, Málnási-Csizmadia, Vértessy: "Kinetic mechanism of human dUTPase, an essential nucleotide pyrophosphatase enzyme." dans: The Journal of biological chemistry, Vol. 282, Issue 46, pp. 33572-82, (2007) (PubMed).

Détail du antigène

Antigène: Deoxyuridine Triphosphatase (DUT)

Autre désignation: DUT

Sujet: DUT is an essential enzyme of nucleotide metabolism. This protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death. This gene encodes an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death. Alternative splicing of this gene leads to different isoforms that localize to either the mitochondrion or nucleus. A related pseudogene is located on chromosome 19.
Alias Symbols: FLJ20622, dUTPase
Protein Interaction Partner: GDI2, NUDT18, PLEKHF2, C19orf25, MRPL14, LEMD3, UBL4A, RPL38, UBC, CUL3, ESRRG, ESRRA, ESR1, SPATA2, DUT, PPARD, PPARA, CDK1,
Protein Size: 252

Poids moléculaire: 19 kDa

ID gène: 1854

NCBI Accession: NM_001025248, NP_001020419

UniProt: P33316