Atp5f1b (C-Term) anticorps

N° du produit ABIN2783269

Cet anticorps Lapin Polyclonal détecte spécifiquement Atp5f1b dans WB, IHC et IP. Il présente une réactivité avec des échantillons de Souris, Humain, Rat, Poisson zèbre (Danio rerio), Saccharomyces cerevisiae, Lapin, Cheval, Cobaye, Chien et Boeuf (Vache). Il a été cité dans 3+ publications.

Aperçu rapide pour Atp5f1b (C-Term) anticorps (ABIN2783269)

Antigène: Atp5f1b

Reactivité: Souris, Humain, Rat, Poisson zèbre (Danio rerio), Saccharomyces cerevisiae, Lapin, Cheval, Cobaye, Chien, Boeuf (Vache)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Inconjugué

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Détail du produit

Épitope: C-Term

Fonction: ATP5F1B Antibody - C-terminal region

Séquence: MGKLVPLKET IKGFQQILAG EYDHLPEQAF YMVGPIEEAV AKADKLAEEH

Homologie: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 77%, Zebrafish: 79%

Attributs du produit: This is a rabbit polyclonal antibody against ATP5B. It was validated on Western Blot using a cell lysate as a positive control. Catalog ABIN973616 can be used a blocking peptide for this product.

Purification: Protein A purified

Immunogène: The immunogen is a synthetic peptide directed towards the C terminal region of human ATP5B

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP48186-100UG

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: prevent freeze-thaw cycles

Stock: 4 °C,-20 °C

Stockage commentaire: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Référence

Usui, Kajita, Kajita, Mori, Hanamoto, Ikeda, Okada, Taguchi, Kitada, Morita, Sasaki, Kitamura, Sato, Kojima, Ishizuka: "Elevated mitochondrial biogenesis in skeletal muscle is associated with testosterone-induced body weight loss in male mice." dans: FEBS letters, Vol. 588, Issue 10, pp. 1935-41, (2014) (PubMed).

Rubel, Schisler, Hamlett, DeKroon, Gautel, Alzate, Patterson: "Diggin' on u(biquitin): a novel method for the identification of physiological E3 ubiquitin ligase substrates." dans: Cell biochemistry and biophysics, Vol. 67, Issue 1, pp. 127-38, (2013) (PubMed).

Teodoro, Duarte, Gomes, Varela, Peixoto, Rolo, Palmeira: "Berberine reverts hepatic mitochondrial dysfunction in high-fat fed rats: a possible role for SirT3 activation." dans: Mitochondrion, Vol. 13, Issue 6, pp. 637-46, (2013) (PubMed).

Détail du antigène

Antigène: Atp5f1b

Sujet:

Targeted Subunit: beta, mitochondrial

Background Information: This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the beta subunit of the catalytic core.

Gene Name: ATP synthase F1 subunit beta

Alternative Symbols: ATP5B, ATPMB, ATPSB, HEL-S-271

Protein Name: ATP synthase subunit beta, mitochondrial

Poids moléculaire: 57 kDa

ID gène: 506

NCBI Accession: NP_001677

UniProt: P06576