NSUN2 anticorps (C-Term)

N° du produit ABIN2783512

Cité dans 1 publication. Cet anticorps anti-NSUN2 Polyclonal Lapin (ABIN2783512) détecte spécifiquement NSUN2 dans WB. L’anticorps est réactif avec des échantillons de Humain, Souris, Rat, Boeuf (Vache), Chien, Cheval, Cobaye, Lapin et Porc.

Aperçu rapide pour NSUN2 anticorps (C-Term) (ABIN2783512)

Antigène: NSUN2 (NOP2/Sun Domain Family, Member 2 (NSUN2))

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Chien, Cheval, Cobaye, Lapin, Porc

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NSUN2 est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-NSUN2 anticorps

Épitope: C-Term

Fonction: NSUN2 Antibody - C-terminal region

Séquence: FINSRIITVS MEDVKILLTQ ENPFFRKLSS ETYSQAKDLA KGSIVLKYEP

Homologie: Cow: 100%, Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 93%, Rat: 100%

Attributs du produit: This is a rabbit polyclonal antibody against NSUN2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogène: The immunogen is a synthetic peptide directed towards the C terminal region of human NSUN2

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8106631

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: prevent freeze-thaw cycles

Stock: 4 °C,-20 °C

Stockage commentaire: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Références pour anticorps anti-NSUN2 (ABIN2783512)

Blanco, Bandiera, Popis, Hussain, Lombard, Aleksic, Sajini, Tanna, Cortés-Garrido, Gkatza, Dietmann, Frye: "Stem cell function and stress response are controlled by protein synthesis." dans: Nature, Vol. 534, Issue 7607, pp. 335-40, (2016) (PubMed).

Détails sur NSUN2

Antigène: NSUN2 (NOP2/Sun Domain Family, Member 2 (NSUN2))

Autre désignation: NSUN2

Sujet:

Background Information: Maturation of cytoplasmic tRNAs includes splicing of introns, which are located 1 nucleotide 3-prime from the anticodon in all intron-containing tRNA genes. In tRNA-leu(CAA), the first position of the anticodon, C34, is converted to 5-methylcytosine, a modification necessary to stabilize the anticodon-codon pairing and correctly translate the mRNA. NSUN2 is a methyltransferase that catalyzes the intron-dependent formation of 5-methylcytosine at C34 of tRNA-leu(CAA).Maturation of cytoplasmic tRNAs includes splicing of introns, which are located 1 nucleotide 3-prime from the anticodon in all intron-containing tRNA genes. In tRNA-leu(CAA), the first position of the anticodon, C34, is converted to 5-methylcytosine, a modification necessary to stabilize the anticodon-codon pairing and correctly translate the mRNA. NSUN2 encodes a methyltransferase that catalyzes the intron-dependent formation of 5-methylcytosine at C34 of tRNA-leu(CAA) (Brzezicha et al., 2006 [PubMed 17071714]).[supplied by OMIM].

Gene Name: NOP2/Sun domain family, member 2

Alternative Symbols: MISU, MRT5, SAKI, TRM4

Protein Name: tRNA (cytosine(34)-C(5))-methyltransferase

Poids moléculaire: 86 kDa

ID gène: 54888

NCBI Accession: NP_060225

UniProt: Q08J23