UBA3 anticorps (N-Term)

N° du produit ABIN2784205

L’anticorps Lapin Polyclonal anti-UBA3 a été validé pour WB et IHC. Il convient pour détecter UBA3 dans des échantillons de Humain, Souris, Rat, Boeuf (Vache), Chien, Poisson zèbre (Danio rerio), Cheval, Porc et Lapin.

Aperçu rapide pour UBA3 anticorps (N-Term) (ABIN2784205)

Antigène: UBA3 (Ubiquitin-Like Modifier Activating Enzyme 3 (UBA3))

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Chien, Poisson zèbre (Danio rerio), Cheval, Porc, Lapin

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp UBA3 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-UBA3 anticorps

Épitope: N-Term

Séquence: EGRWNHVKKF LERSGPFTHP DFEPSTESLQ FLLDTCKVLV IGAGGLGCEL

Homologie: Cow: 100%, Dog: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 86%

Attributs du produit: This is a rabbit polyclonal antibody against UBA3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogène: The immunogen is a synthetic peptide directed towards the N terminal region of human UBA3

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator.

Commentaires:

Antigen size: 463 AA

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Détails sur UBA3

Antigène: UBA3 (Ubiquitin-Like Modifier Activating Enzyme 3 (UBA3))

Autre désignation: UBA3

Sujet: UBA3 is a catalytic subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. UBA3 down-regulates steroid receptor activity. UBA3 is necessary for cell cycle progression.The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme associates with AppBp1, an amyloid beta precursor protein binding protein, to form a heterodimer, and then the enzyme complex activates NEDD8, a ubiquitin-like protein, which regulates cell division, signaling and embryogenesis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Alias Symbols: DKFZp566J164, MGC22384, UBE1C, hUBA3
Protein Interaction Partner: UBA3, UBC, IMPA2, CPNE2, NEDD8, CAMK1D, NSFL1C, DCPS, KDM1A, PAPSS1, NAE1, XPO1, PCYT2, CAPN2, AHCY, ACAT2, NT5DC1, NUDCD2, TXNDC17, WDR61, UFC1, DSTN, VCL, UBE2H, PRDX2, TWF1, UBE2M, UIMC1, CHD3,
Protein Size: 463

Poids moléculaire: 52 kDa

ID gène: 9039

NCBI Accession: NM_003968, NP_003959

UniProt: Q8TBC4