HYAL1 anticorps (N-Term)

N° du produit ABIN2784343

Cet validé indépendamment anticorps Lapin Polyclonal détecte spécifiquement HYAL1 dans WB et IHC. Il présente une réactivité envers Humain, Souris, Rat, Boeuf (Vache), Chien, Cobaye, Cheval et Lapin.

Aperçu rapide pour HYAL1 anticorps (N-Term) (ABIN2784343)

Antigène: HYAL1 (Hyaluronidase-1 (HYAL1))

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Chien, Cobaye, Cheval, Lapin

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp HYAL1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-HYAL1 anticorps

Épitope: N-Term

Séquence: TIFYSSQLGT YPYYTPTGEP VFGGLPQNAS LIAHLARTFQ DILAAIPAPD

Homologie: Cow: 86%, Dog: 85%, Guinea Pig: 86%, Horse: 93%, Human: 100%, Mouse: 92%, Rabbit: 93%, Rat: 92%

Attributs du produit: This is a rabbit polyclonal antibody against HYAL1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogène: The immunogen is a synthetic peptide directed towards the N terminal region of human HYAL1

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator.

Commentaires:

Antigen size: 435 AA

Restrictions: For Research Use only

Independent Validation (IHC)

Validation #100034 (Immunohistochemistry)

by: University of Manitoba, Max Rady College of Medicine Biochemistry and Medical Genetics, Department of Biochemistry and Medical Genetics

No.: #100034

Date: 16.08.2016

Antigène: HYAL1 antibody - N-terminal region

Numéro du lot: QC23783

Application validée: Immunohistochemistry

Contrôle positif: Testes of Hyal1 +/+ mouse

Contrôle négative: Testes of Hyal1 -/- mouse

Conclusion: ABIN2784343 does detect mouse HYAL1 by immunohistochemistry in paraffin sections. The immunohistochemistry is based on increased signal in wild type mouse testes compared to Hyal1-/- mouse testes.

Validation Images

Testes of Hyal1 -/- mouse (A) or Hyal1 +/+ mouse (B) stained with ABIN2784342 as described in the protocol.

Protocole

Anticorps primaire: ABIN2784343

Anticorps secondaire: Goat-anti-rabbit AF568

Full Protocol:

• Deparafinize and rehydrate:
• xylene (I & II) – 2x 5min
• 100% Ethanol (I & II) – 2x 5min
• 95% Ethanol (I & II) – 2x 3min
• 70% Ethanol (I) – 3min
• 50% Ethanol (I) – 3min
• ddH₂O – 3min
• Antigen retrieval:
• Immerse slides in citrate buffer (10mM Sodium citrate at pH6.0) preheated to 95°C.
• Incubate at 95°C for 20min.
• Cool down at RT for 20min.
• Wash twice with PBS for 5min.
• Immunostaining:
• Block in blocking solution (prepare 3% BSA in TBST) at RT for 60min.
• Incubate sections with primary Hyaluronidase-1 (HYAL1) (N-Term) antibody ABIN2784343 diluted 1:200 in blocking solution.
• Incubate at 4°C overnight.
• Wash slides three times with TBST 5min.
• Incubate sections with goat-anti-rabbit AF568 secondary antibody conjugate diluted 1:500 in blocking solution.
• Incubate at RT for 1h.
• Wash slides in TBST - 1x 5min at RT.
• Wash three times with H₂O for 5min.
• Incubated in Hoechst solution diluted 1:100.000 for 8-9min.
• Wash twice with H₂O for 5min.
• Mounted with ProLong Gold (ThermoFisher, P36931) solution.

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Détails sur HYAL1

Antigène: HYAL1 (Hyaluronidase-1 (HYAL1))

Autre désignation: HYAL1

Sujet: HYAL1 is a lysosomal hyaluronidase. Hyaluronidases intracellularly degrade hyaluronan, one of the major glycosaminoglycans of the extracellular matrix. Hyaluronan is thought to be involved in cell proliferation, migration and differentiation. This enzyme is active at an acidic pH and is the major hyaluronidase in plasma. Mutations in this gene are associated with mucopolysaccharidosis type IX, or hyaluronidase deficiency. This gene is one of several related genes in a region of chromosome 3p21.3 associated with tumor suppression. Multiple transcript variants encoding different isoforms have been found for this gene.This gene encodes a lysosomal hyaluronidase. Hyaluronidases intracellularly degrade hyaluronan, one of the major glycosaminoglycans of the extracellular matrix. Hyaluronan is thought to be involved in cell proliferation, migration and differentiation. This enzyme is active at an acidic pH and is the major hyaluronidase in plasma. Mutations in this gene are associated with mucopolysaccharidosis type IX, or hyaluronidase deficiency. The gene is one of several related genes in a region of chromosome 3p21.3 associated with tumor suppression. Multiple transcript variants encoding different isoforms have been found for this gene.
Alias Symbols: HYAL-1, LUCA1, MGC45987, NAT6
Protein Interaction Partner: UBC, COL2A1,
Protein Size: 435

Poids moléculaire: 45 kDa

ID gène: 3373

NCBI Accession: NM_153282, NP_695014

UniProt: Q12794

Pathways: Glycosaminoglycan Metabolic Process