DBT anticorps (N-Term)

N° du produit ABIN2784728

Cet anticorps anti-DBT est un anticorps Lapin Polyclonal détectant DBT dans WB et IHC. Adapté pour Humain, Souris, Rat, Boeuf (Vache), Chien, Cobaye, Cheval, Lapin, Poisson zèbre (Danio rerio) et Saccharomyces cerevisiae.

Aperçu rapide pour DBT anticorps (N-Term) (ABIN2784728)

Antigène: DBT (Dihydrolipoamide Branched Chain Transacylase E2 (DBT))

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Chien, Cobaye, Cheval, Lapin, Poisson zèbre (Danio rerio), Saccharomyces cerevisiae

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp DBT est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-DBT anticorps

Épitope: N-Term

Séquence: NYVCFFGYPS FKYSHPHHFL KTTAALRGQV VQFKLSDIGE GIREVTVKEW

Homologie: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 83%, Zebrafish: 93%

Attributs du produit: This is a rabbit polyclonal antibody against DBT. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogène: The immunogen is a synthetic peptide directed towards the N terminal region of human DBT

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator.

Commentaires:

Antigen size: 482 AA

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Détails sur DBT

Antigène: DBT (Dihydrolipoamide Branched Chain Transacylase E2 (DBT))

Autre désignation: DBT

Sujet: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO2. It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3).The branched-chain alpha-keto acid dehydrogenase complex (BCKD) is an inner-mitochondrial enzyme complex involved in the breakdown of the branched-chain amino acids isoleucine, leucine, and valine. The BCKD complex is thought to be composed of a core of 24 transacylase (E2) subunits, and associated decarboxylase (E1), dehydrogenase (E3), and regulatory subunits. This gene encodes the transacylase (E2) subunit. Mutations in this gene result in maple syrup urine disease, type 2. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: BCATE2, E2, E2B, MGC9061
Protein Interaction Partner: HUWE1, UBC, PARK2, IFIT2, ABCC1, DLD, AGO4, AGO3, SRRM2, USP16, ADI1,
Protein Size: 482

Poids moléculaire: 46 kDa

ID gène: 1629

NCBI Accession: NM_001918, NP_001909

UniProt: P11182