POLI anticorps (N-Term)

N° du produit ABIN2784869

L’anticorps anti-POLI Polyclonal Lapin est utilisé pour la détection de POLI dans des échantillons de Humain, Souris, Rat, Boeuf (Vache), Chien, Cobaye, Cheval, Poisson zèbre (Danio rerio) et Lapin. Il a été validé pour WB.

Aperçu rapide pour POLI anticorps (N-Term) (ABIN2784869)

Antigène: POLI (Polymerase (DNA Directed) iota (POLI))

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Chien, Cobaye, Cheval, Poisson zèbre (Danio rerio), Lapin

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp POLI est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-POLI anticorps

Épitope: N-Term

Fonction: POLI Antibody - N-terminal region

Séquence: PVVERLGFDE NFVDLTEMVE KRLQQLQSDE LSAVTVSGHV YNNQSINLLD

Homologie: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 86%

Attributs du produit: This is a rabbit polyclonal antibody against POLI. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogène: The immunogen is a synthetic peptide directed towards the N terminal region of human POLI

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP52299-100UG

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: prevent freeze-thaw cycles

Stock: 4 °C,-20 °C

Stockage commentaire: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Détails sur POLI

Antigène: POLI (Polymerase (DNA Directed) iota (POLI))

Autre désignation: POLI

Sujet:

Background Information: POLI is an error-prone DNA polymerase specifically involved in DNA repair.POLI plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls.POLI favors Hoogsteen base-pairing in the active site.POLI inserts the correct base with high-fidelity opposite an adenosine template.POLI exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine.POLI may play a role in hypermutation of immunogobulin genes.POLI forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity.

Gene Name: Polymerase (DNA directed) iota

Alternative Symbols: eta2, RAD30B, RAD3OB

Protein Name: DNA polymerase iota

Poids moléculaire: 83

ID gène: 11201

NCBI Accession: NP_009126

UniProt: Q9UNA4

Pathways: Réparation de l'ADN