POLI anticorps (N-Term)

N° du produit ABIN2784869

Détail du produit anti-POLI anticorps

Antigène: POLI (Polymerase (DNA Directed) iota (POLI))

Épitope: N-Term

Reactivité: Humain, Rat, Souris, Boeuf (Vache), Chien, Cobaye, Cheval, Poisson zèbre (Danio rerio), Lapin, Saccharomyces cerevisiae

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp POLI est non-conjugé

Application: Western Blotting (WB)

Séquence: PVVERLGFDE NFVDLTEMVE KRLQQLQSDE LSAVTVSGHV YNNQSINLLD

Homologie: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 82%, Zebrafish: 86%

Attributs du produit: This is a rabbit polyclonal antibody against POLI. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogène: The immunogen is a synthetic peptide directed towards the N terminal region of human POLI

Information d'application

Indications d'application: Optimal working dilutions should be determined experimentally by the investigator.

Commentaires:

Antigen size: 740 AA

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Détails sur POLI

Antigène: POLI (Polymerase (DNA Directed) iota (POLI))

Autre désignation: POLI (POLI Produits)

Synonymes: anticorps poli, anticorps wu:fc27h11, anticorps zgc:110185, anticorps GB12093, anticorps NV16187, anticorps RAD30B, anticorps RAD3OB, anticorps Rad30b, anticorps DNA polymerase iota, anticorps polymerase (DNA directed) iota, anticorps polymerase (DNA directed), iota, anticorps POLI, anticorps poli, anticorps LOC551219, anticorps AFUA_4G11140, anticorps LOC5568276, anticorps Pol-iota, anticorps Poli

Sujet: POLI is an error-prone DNA polymerase specifically involved in DNA repair.POLI plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls.POLI favors Hoogsteen base-pairing in the active site.POLI inserts the correct base with high-fidelity opposite an adenosine template.POLI exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine.POLI may play a role in hypermutation of immunogobulin genes.POLI forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity.
Alias Symbols: RAD30B, RAD3OB
Protein Interaction Partner: TMEM239, TRIM39, ZBTB44, UBC, TRAF6, DAZAP2, RNF11, TRIM23, POLH, PCNA, APP, REV1, XRCC1, UBB, UBA52, CALCOCO2, DVL2,
Protein Size: 740

Poids moléculaire: 83 kDa

ID gène: 11201

NCBI Accession: NM_007195, NP_009126

UniProt: Q9UNA4

Pathways: Réparation de l'ADN