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CCDC42 anticorps (Middle Region)

CCDC42 Reactivité: Humain, Souris, Rat, Cobaye, Cheval, Lapin, Boeuf (Vache), Chien WB Hôte: Lapin Polyclonal unconjugated
N° du produit ABIN2785068
  • Antigène Voir toutes CCDC42 Anticorps
    CCDC42 (Coiled-Coil Domain Containing 42 (CCDC42))
    Épitope
    • 15
    • 8
    • 7
    • 6
    • 6
    • 6
    • 4
    • 1
    Middle Region
    Reactivité
    Humain, Souris, Rat, Cobaye, Cheval, Lapin, Boeuf (Vache), Chien
    Hôte
    • 38
    Lapin
    Clonalité
    • 38
    Polyclonal
    Conjugué
    • 8
    • 5
    • 5
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp CCDC42 est non-conjugé
    Application
    • 32
    • 22
    • 14
    • 13
    • 13
    • 4
    • 3
    Western Blotting (WB)
    Séquence
    ENSEFEEIHE VIARYKTLVS MHHDLMQSAQ EGQEKIEHAK ARLARYMEEK
    Homologie
    Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 93%, Rat: 93%
    Attributs du produit
    This is a rabbit polyclonal antibody against Ccdc42. It was validated on Western Blot.
    Purification
    Affinity Purified
    Immunogène
    The immunogen is a synthetic peptide directed towards the middle region of Rat Ccdc42
    Top Product
    Discover our top product CCDC42 Anticorps primaire
  • Indications d'application
    Optimal working dilutions should be determined experimentally by the investigator.
    Commentaires

    Antigen size: 316 AA

    Restrictions
    For Research Use only
  • Validation #103398 (Immunocytochemistry)
    'Independent Validation' signe
    by
    Developmental Biology, Johann-Friedrich-Blumenbach-Institute for Zoology and Anthropology, Georg-August-University of Göttingen
    No.
    #103398
    Date
    12.10.2018
    Antigène
    CCDC42
    Numéro du lot
    QC28887-41199
    Application validée
    Immunocytochemistry
    Contrôle positif
    NIH/3T3 cells transfected with Cddc42-mCherry expression vector
    Contrôle négative
    Non-transfected NIH/3T3 cells
    Conclusion

    Passed. ABIN2785068 specifically detects Ccdc42-Cherry when exogenously expressed. Background staining is low.

    'Independent Validation' signe
    Validation Images
    Protocole
    Anticorps primaire
    ABIN2785068
    Anticorps secondaire
    goat anti-rabbit 488 antibody (MoBiotec, MFP-A1008, lot 2806F058)
    Full Protocol
    • Grow NIH/3T3 cells (ATCC, CRL-1658) in DMEM+GlutaMAX (Gibco, 10567-014, lot 1922818) supplemented with fetal bovine serum (Gibco, 10500-064, lot 08FO477K) and Pen/Strep (Gibco, 15140-122, lot 1924797) at 37°C and 5% CO2 to 70% confluency.
    • Transfect cells with Ccdc42 tagged mCherry (Ccdc42-Cherry) expression vector using EndofectinMax (GeneCopoeia) following the manufacturer´s instructions.
    • Grow cells for 24h.
    • Fix cells in methanol for 10min at -20°C followed by incubation in 0.3% Triton X-100 for 10min at 4°C.
    • Block cells in PBS containing 1% bovine serum albumin and 0.5% Tween-20 (PBT) for 1h at RT.
    • Incubate cells with primary rabbit anti-Ccdc42 antibody (antibodies-online, ABIN2785068, QC28887-41199) diluted 1:100 in PBS for 1h at 37°C.
    • Wash cells 3x 5min with 1x PBS.
    • Incubate cells with secondary goat anti-rabbit 488 antibody (MoBiotec, MFP-A1008, lot 2806F058) diluted 1:300 for 1h at 37°C in PBS.
    • DNA was counterstained with DAPI (4’, 6-Diamidino-2-phenylindole; Sigma, D-9542).
    • Image acquisition on Zeiss LSM 510 confocal microscope and processing using Adobe Photoshop 5.0.
    Notes
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -20 °C
    Stockage commentaire
    For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
  • Wu, Yu, Liu, Wang, Huang, Lu, Chen, Li, Liu: "Essential Role of CFAP53 in Sperm Flagellum Biogenesis." dans: Frontiers in cell and developmental biology, Vol. 9, pp. 676910, (2021) (PubMed).

    Tapia Contreras, Hoyer-Fender: "CCDC42 Localizes to Manchette, HTCA and Tail and Interacts With ODF1 and ODF2 in the Formation of the Male Germ Cell Cytoskeleton." dans: Frontiers in cell and developmental biology, Vol. 7, pp. 151 (PubMed).

  • Antigène
    CCDC42 (Coiled-Coil Domain Containing 42 (CCDC42))
    Autre désignation
    Ccdc42 (CCDC42 Produits)
    Synonymes
    anticorps RGD1565925, anticorps CCDC42A, anticorps A530001H01Rik, anticorps coiled-coil domain containing 42, anticorps Ccdc42, anticorps CCDC42
    Sujet
    The function of this protein remains unknown.
    Alias Symbols: RGD1565925
    Protein Size: 316
    Poids moléculaire
    34 kDa
    ID gène
    303234
    NCBI Accession
    NM_001107009, NP_001100479
    UniProt
    D3ZAZ8
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