GCLC anticorps (Middle Region)

N° du produit ABIN2785780

Cet anticorps Lapin Polyclonal détecte spécifiquement GCLC dans WB et IHC. Il présente une réactivité avec des échantillons de Humain, Souris, Rat, Boeuf (Vache), Poisson zèbre (Danio rerio), Lapin, Cobaye, Chien, Cheval et Saccharomyces cerevisiae. Il a été cité dans 2+ publications.

Aperçu rapide pour GCLC anticorps (Middle Region) (ABIN2785780)

Antigène: GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Poisson zèbre (Danio rerio), Lapin, Cobaye, Chien, Cheval, Saccharomyces cerevisiae

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GCLC est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-GCLC anticorps

Épitope: Middle Region

Fonction: GCLC Antibody - middle region

Séquence: RISKSRYDSI DSYLSKCGEK YNDIDLTIDK EIYEQLLQEG IDHLLAQHVA

Homologie: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 100%, Zebrafish: 100%

Attributs du produit: This is a rabbit polyclonal antibody against GCLC. It was validated on Western Blot.

Purification: Affinity Purified

Immunogène: The immunogen is a synthetic peptide directed towards the middle region of human GCLC

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under AAP54577-100UG

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: prevent freeze-thaw cycles

Stock: 4 °C,-20 °C

Stockage commentaire: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Références pour anticorps anti-GCLC (ABIN2785780)

Ramani, Tomasi, Yang, Ko, Lu: "Mechanism and significance of changes in glutamate-cysteine ligase expression during hepatic fibrogenesis." dans: The Journal of biological chemistry, Vol. 287, Issue 43, pp. 36341-55, (2012) (PubMed).

Bardag-Gorce, Oliva, Lin, Li, French, French: "Proteasome inhibitor up regulates liver antioxidative enzymes in rat model of alcoholic liver disease." dans: Experimental and molecular pathology, Vol. 90, Issue 1, pp. 123-30, (2011) (PubMed).

Détails sur GCLC

Antigène: GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))

Autre désignation: GCLC

Sujet:

Background Information: Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. GCLC is the catalytic subunit of 637 amino acids with a calculated molecular weight of 72.773 kDa. Deficiency of gamma-glutamylcysteine synthetase in human is associated with enzymopathic hemolytic anemia.

Gene Name: Glutamate-cysteine ligase, catalytic subunit

Alternative Symbols: GCL, GCS, GLCL, GLCLC

Protein Name: Glutamate--cysteine ligase catalytic subunit

Poids moléculaire: 73 kDa

ID gène: 2729

NCBI Accession: NP_001489

UniProt: P48506

Pathways: Cell RedoxHomeostasis