LEF1 anticorps (N-Term)

N° du produit ABIN2792635

L’anticorps Lapin Polyclonal anti-LEF1 a été validé pour WB. Il convient pour détecter LEF1 dans des échantillons de Humain, Souris, Rat, Chien, Cobaye, Boeuf (Vache) et Cheval.

Aperçu rapide pour LEF1 anticorps (N-Term) (ABIN2792635)

Antigène: LEF1 (Lymphoid Enhancer-Binding Factor 1 (LEF1))

Reactivité: Humain, Souris, Rat, Chien, Cobaye, Boeuf (Vache), Cheval

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp LEF1 est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-LEF1 anticorps

Épitope: N-Term

Séquence: VARQAQTSQE PYHDKAREHP DDGKHPDGGL YNKGPSYSSY SGYIMMPNMN

Réactivité croisée: Boeuf (Vache), Humain, Souris, Porc, Lapin, Rat (Rattus)

Homologie: Cow: 92%, Dog: 92%, Guinea Pig: 85%, Horse: 100%, Human: 100%, Mouse: 85%, Rat: 92%

Attributs du produit: This is a rabbit polyclonal antibody against LEF1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogène: The immunogen is a synthetic peptide directed towards the N terminal region of human LEF1

Information d'application

Indications d'application: WB Suggested Anti-LEF1 Antibody Titration: 0.2-1 µg/mL
ELISA Titer: 1:312500
Positive Control: Hela cell lysate.
Optimal working dilutions should be determined experimentally by the investigator.

Commentaires:

Antigen size: 399 AA

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -20 °C

Stockage commentaire: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Détails sur LEF1

Antigène: LEF1 (Lymphoid Enhancer-Binding Factor 1 (LEF1))

Autre désignation: LEF1

Sujet: Lymphoid enhancer-binding factor-1 (LEF1) is a 48-kD nuclear protein that is expressed in pre-B and T cells. It binds to a functionally important site in the T-cell receptor-alpha (TCRA) enhancer and confers maximal enhancer activity. LEF1 belongs to a family of regulatory proteins that share homology with high mobility group protein-1 (HMG1). Lymphoid enhancer-binding factor-1 (LEF1) is a 48-kD nuclear protein that is expressed in pre-B and T cells. It binds to a functionally important site in the T-cell receptor-alpha (TCRA, MIM 186880) enhancer and confers maximal enhancer activity. LEF1 belongs to a family of regulatory proteins that share homology with high mobility group protein-1 (HMG1, MIM 163905).[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: DKFZp586H0919, TCF1ALPHA, LEF-1, TCF10, TCF7L3
Protein Interaction Partner: RNF138, UBC, RNF14, NLK, NARF, STAT5A, PITX2, CTNNB1, CDX1, DPYSL2, nrarp, FHIT, MITF, HINT1, TLE2, TLE1, SETD8, MYB, E2F1, HDAC1, ZBTB3, DACT1, DAZAP2, ALYREF, PIAS4, ALX4, SMAD3, SUMO2, EP300, SMAD1, SMAD2, SMAD4, KPNA1, KPNA2, UBTF, RUNX2, NOTCH1, RUVB
Protein Size: 399

Poids moléculaire: 44 kDa

ID gène: 51176

NCBI Accession: NM_016269, NP_057353

UniProt: Q9UJU2

Pathways: Signalisation WNT, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Hormone Metabolic Process, Nuclear Hormone Receptor Binding, Chromatin Binding