DKK3 anticorps (N-Term)

N° du produit ABIN2798864

L’anticorps Lapin Polyclonal anti-DKK3 a été validé pour WB et IHC. Il convient pour détecter DKK3 dans des échantillons de Humain.

Aperçu rapide pour DKK3 anticorps (N-Term) (ABIN2798864)

Antigène: DKK3

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp DKK3 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-DKK3 anticorps

Épitope: AA 15-45, N-Term

Fonction: Rabbit Anti-Human DKK3 (N-term) Antibody

Immunogène: This DKK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15-45 amino acids from the N-terminal region of human DKK3.

Isotype: Ig Fraction

Information d'application

Indications d'application: Western Blot, Immunohistochemistry
Recommended Dilutions
WB: 1:1000, IHC: 1:50-100Western blot analysis of lysates from 293, 293T, A549, HL-60, MCF-7 cell line and mouse brain tissue lysate (from left to right), using DKK3 Antibody (A30), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry, clinical relevance has not been evaluated.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.500 mg/mL

Stock: 4 °C,-20 °C

Stockage commentaire: 2-8°C (short-term), -20°C (long-term)

Détails sur DKK3

Antigène: DKK3

Sujet:

Target Description: DKK3, like DKK1, DKK2, and DKK4, possesses an N-terminal signal peptide and 2 conserved cysteine-rich domains, which are separated by a linker region and contain 10 cysteine residues each. The second cysteine region has a putative lipid-binding function that may facilitate WNT/DKK interactions at the plasma membrane. The linker region contains 50 to 55 amino acids in DKK1, DKK2, and DKK4, whereas in DKK3 it contains only 12 amino acids. All DKKs have several potential sites for cleavage by furin-type proteases. Northern blot analysis revealed wide expression of the DKK3 transcript, with highest expression in heart, brain, and spinal cord. In situ hybridization reveals highest expression in mouse brain, eye, and heart.

Gene Symbol: DKK3

Poids moléculaire: 38390 Da

ID gène: 27122

UniProt: Q9UBP4