ERAS anticorps (N-Term)

N° du produit ABIN2800622

L’anticorps Lapin Polyclonal anti-ERAS a été validé pour WB, IHC et IF. Il convient pour détecter ERAS dans des échantillons de Humain.

Aperçu rapide pour ERAS anticorps (N-Term) (ABIN2800622)

Antigène: ERAS (ES cell expressed Ras (ERAS))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ERAS est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: F66

Détail du produit anti-ERAS anticorps

Épitope: AA 51-81, N-Term

Fonction: Rabbit Anti-Human ERAS (N-term) (F66) Antibody

Immunogène: This ERAS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 51-81 amino acids from the N-terminal region of human ERAS.

Isotype: Ig Fraction

Information d'application

Indications d'application: Immunofluorescence, Western Blot, Immunohistochemistry
Recommended Dilutions
IF: 1:100, WB: 1:1000, IHC: 1:10-50Fluorescent confocal image of SY5Y cells stained with ERAS (N-term) (F66) antibody. SY5Y cells were fixed with 4 % PFA (20 min), permeabilized with Triton X-100 (0.2 %, 30 min). Cells were then incubated with 102-12613 ERAS (N-term) (F66) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/mL, 5 min). ERAS immunoreactivity is localized to the plasma membrane of SY5Y cells.Western blot analysis of ERAS Antibody (N-term) (F66) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry, clinical relevance has not been evaluated.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 2.0 mg/mL

Stock: 4 °C,-20 °C

Stockage commentaire: 2-8°C (short-term), -20°C (long-term)

Détails sur ERAS

Antigène: ERAS (ES cell expressed Ras (ERAS))

Autre désignation: ERAS

Sujet:

Target Description: Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Point mutations of several amino acids of human RAS, including gly12, ala59, or glu63, render the protein constitutively active. Embryonic stem cell-expressed Ras (ERAS) has serine, alanine, and asparagine at the positions corresponding to gly12, ala59, and glu63 of human RAS, suggesting that it is constitutively active.The PI3K (phosphoinositide 3-kinase) pathway is important for proliferation, survival and maintenance of pluripotency in ES cells. The PI3K pathway is activated by growth factors and cytokines including insulin and leukaemia inhibitory factor. In addition to these exogenous factors, the PI3K pathway is endogenously activated by the constitutively active Ras family protein ERas (ES cell-expressed Ras). ERas null ES cells maintained pluripotency but show significantly reduced growth and tumorigenicity, which can be rescued by expression of ERas cDNA or by activated phosphatidylinositol 3-hydroxykinase. The transforming oncogene ERAS appears to be important in the tumor-like growth properties of ES cells.

Gene Symbol: ERAS

Poids moléculaire: 25287 Da

ID gène: 3266

UniProt: Q7Z444