PFKM anticorps (N-Term)

N° du produit ABIN2854614

Cet anticorps anti-PFKM est un anticorps Lapin Polyclonal détectant PFKM dans WB. Adapté pour Humain.

Aperçu rapide pour PFKM anticorps (N-Term) (ABIN2854614)

Antigène: PFKM (phosphofructokinase, Muscle (PFKM))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PFKM est non-conjugé

Application: Western Blotting (WB)

Classe de qualité: KO Validated

Détail du produit anti-PFKM anticorps

Épitope: N-Term

Réactivité croisée: Humain, Souris, Rat

Attributs du produit: Rabbit Polyclonal antibody to PFK (muscle) (phosphofructokinase, muscle)
PFK (muscle) antibody [N1N2-2], N-term

Purification: Purified by antigen-affinity chromatography.

Immunogène: Recombinant protein encompassing a sequence within the N-terminus region of human Fructose 6 Phosphate Kinase. The exact sequence is proprietary.

Isotype: IgG

Information d'application

Indications d'application: WB: 1:500-1:3000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Commentaires:

Positive Control: mouse brain , rat brain , HeLa

Validation: KO/KD

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.52 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Détails sur PFKM

Antigène: PFKM (phosphofructokinase, Muscle (PFKM))

Autre désignation: phosphofructokinase, muscle

Sujet: The PFKM gene encodes the muscle isoform of phosphofructokinase (PFK) (ATP:D-fructose-6-phosphate-1-phosphotransferase, EC 2.7.1.11). PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1,6-bisphosphate and is a key regulatory enzyme in glycolysis. Mammalian PFK is a tetramer made up of various combinations of 3 subunits: muscle (PFKM), liver (PFKL, MIM 171860), and platelet (PFKP, MIM 171840), the genes for which are located on chromosomes 12q13, 21q22, and 10p, respectively. The composition of the tetramers differs according to the tissue type. Muscle and liver PFK are a homotetramers of 4M and 4L subunits, respectively. Erythrocytes contain both L and M subunits, which randomly tetramerize to form M4, L4, and M3L, M2L2, and ML3 hybrid forms of the holoenzyme (Vora et al., 1980 [PubMed 6444721], Raben and Sherman, 1995 [PubMed 7550225]).[supplied by OMIM]

Poids moléculaire: 85 kDa

ID gène: 5213

UniProt: P08237

Pathways: Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, L'effet Warburg